萎缩小肠粘膜上皮细胞凋亡及其相关基因表达的研究  

STUDY ON APOPTOSIS AND EXPRESSION OF APOPTOSIS RELATED GENES IN ATROPHIC INTESTINAL MUCOSAL EPITHELIUM

作　　者：谢建新[1] 顾岩[2] 赵淑民[1] 罗宝国[1] 陈丽琏[1] 吴肇汉 左焕琛[1] 

机构地区：[1]上海医科大学人体解剖学教研室 [2]中山医院普外科,上海200032

出　　处：《解剖学杂志》1999年第2期118-123,共6页Chinese Journal of Anatomy

基　　金：国家教委博士点基金!(No.9736);上海市卫生局科技发展基金! (No.97429)资助

摘　　要：目的 :探讨细胞凋亡与小肠粘膜萎缩发生之间的关系。材料和方法 :采用原位末端标记、免疫组化和 RT-PCR的方法 ,对正常小肠粘膜上皮和萎缩小肠粘膜上皮细胞凋亡的发生及其相关基因的表达进行对比研究。结果 :萎缩小肠粘膜上皮细胞凋亡的发生率明显高于正常小肠 ( P<0 .0 5) ;C- MYC在萎缩小肠绒毛顶部异常高表达 ;BAX和 BCL- 2呈弥散分布 ,BCL- 2在萎缩小肠粘膜上皮的表达显著低于正常小肠 ( P<0 .0 5) ,BAX的表达虽无显著性差异 ,但 BCL- 2与 BAX的比值明显降低。萎缩小肠粘膜上皮 bcl- 2的 m RNA表达较正常小肠明显降低 ,而 c-myc的 m RNA表达明显增高。结论 :小肠粘膜萎缩的发生与其细胞凋亡发生率增高有关 ,而细胞凋亡的增多与 bcl-2和 c-Objective:To explore the relationship between apoptosis and intestinal mucosal atrophy. Method: A contrasting study on the occurrence and the distribution of apoptosis and the expression of apoptotic related genes between the normal and the atrophic intestinal mucosal epithelia was made by using the TUNEL(terminal deoxynudeotidy L transfere mediated dUTP biotin nick end ladeling) method, the immunohistochemical staining and reverse transcription PCR(RT PCR) method. Results: The rate of the apoptosis in the atrophic intestinal mucosal epithelia was significantly greater(P<0.05) than that in the normal intestine; the expression of C MYC was unusually increased in the atrophic intestinal villous tops; the distribution of BAX and BCL 2 was spread all over the epithelia and the expression of BCL 2 was significantly reduced (P<0.05) in atrophic intestine as compared with that in the normal intestine; there was no significant difference between the atrophic and the normal intestine in BAX expression, but the ratio of BCL 2 to BAX in the atrophic intestine was sinificantly reduced(P<0.05). Bcl 2 mRNA expression was more significantly reduced in the atrophic intestinal mucosa than that in the normal intestine, but c myc mRNA expression was significantly increased. Conclusion: It demonstrated that the occuring of the intestinal mucosal atrophy was closely related with the increasing of the apoptosis, and the increasing of the apoptosis was closely related with the abnormal expression of apoptosis related genes.

关 键 词：小肠粘膜 萎缩 细胞凋亡 基因表达 上皮细胞 

分 类 号：R361.1[医药卫生—病理学] R574.502[医药卫生—基础医学]