萎缩小肠粘膜上皮细胞凋亡及增殖细胞核抗原表达变化的研究  

STUDY ON CHANGES OF APOPTOSIS AND EXPRESSION OF PCNA IN ATROPHIC INTESTINAL MUCOSAL EPITHELIA*

作　　者：谢建新[1] 顾岩 赵淑民[1] 罗宝国[1] 陈丽琏[1] 吴肇汉 左焕琛[1] 

机构地区：[1]上海医科大学人体解剖学教研室 [2]中山医院普外科,上海200032

出　　处：《解剖学杂志》1999年第2期124-127,共4页Chinese Journal of Anatomy

基　　金：国家教委博士点基金!(No.9736 );上海市卫生局科技发展基金! (No.97429)资助

摘　　要：目的 :探讨小肠粘膜萎缩的发生机制。材料和方法 :采用原位末端标记和免疫组化的方法 ,对正常和萎缩小肠粘膜上皮细胞凋亡的发生、分布及增殖细胞核抗原 ( PCNA)的表达进行对比研究。结果 :凋亡细胞主要位于肠绒毛的顶部 ,萎缩小肠粘膜上皮细胞凋亡的发生率明显高于正常小肠 ( P<0 .0 5) ;而 PCNA阳性表达细胞主要位于肠腺隐窝区 ,萎缩小肠粘膜上皮细胞 PCNA的阳性计数较正常小肠显著降低 ( P<0 .0 5)。结论Objective: In order to elucidate the mechanism of intestinal mucosal atrophy. Method: A contrasting study on the occurrence and distribution of apoptosis and the expression of proliferating cell nuclear antigen was made between the normal and the atrophic intestinal mucosal epithelia by using the TUNEL(terminal deoxynudeotidy L transfere mediated dUTP biotin nick end ladeling) method and the immunohistochemical staining. Results: The apoptotic cells were located mainly in the intestinal villous tops; the rate of apoptosis in the atrophic intestinal mucosal epithelia was significantrly greater(P<0.05) than that in the normal intestine, but PCNA immunopositive cells were located mainly in the intestinal crypt; the expression of PCNA was significantly reduced(P<0.05) in the atrophic intestine as compared with that in the normal intestine. Conclusion:It demonstrated that the occurrence of the intestinal mucosal atrophy was the result of the increasing apoptosis in the intestinal villous tops and the decreasing of proliferation in the intestinal crypt.

关 键 词：小肠粘膜 萎缩 细胞凋亡 上皮细胞 PCNA 

分 类 号：R361.1[医药卫生—病理学] R574.502[医药卫生—基础医学]