海洋青鳉鱼专用型基因芯片的设计及其在生态毒理学上的应用研究  

Design of marine medaka (Oryzias melastigma) functional specific cDNA microarray and its ecotoxicological application

作　　者：陈瑶[1] 方志俊[1,2] 郑金树[2] 张琦[2] 曾志雄[2] 胡绍燊[1] 杨梦甦[1,2] 

机构地区：[1]深圳市药用生物芯片重点实验室、香港城市大学深圳研究院,广东深圳518057 [2]香港城市大学生物与化学系,中国香港

出　　处：《台湾海峡》2010年第3期359-366,共8页Journal of Oceanography In Taiwan Strait

基　　金：深圳市科技计划资助项目

摘　　要：以生物芯片技术为核心,结合生态毒理学与功能基因组学的方法,制备了海洋青鳉鱼专用型生物芯片,包含180个与细胞分裂、解毒反应、缺氧反应、氧化应激反应、凋亡、生长和发育、性别决定和性腺分化以及生殖激素分泌等功能相关的基因.我们提取了雌性海洋青鳉鱼成体在正常培养和缺氧处理12周后的肝脏和卵巢组织总RNA,利用自制的基因芯片进行了基因表达谱分析,结果显示海洋青鳉鱼在缺氧压力环境下,肝脏和卵巢组织的差异性表达基因分别检测出9个和6个,表明应用此类自制良好的芯片可有效获得海洋青鳉鱼的组织特异性基因表达图谱,也将加强对海洋缺氧状态、氧化应激等反应的分子生物学机制的理解.本研究对于识别异常环境状态下的基因表达模式和组织特异性标志物,进一步开展监测海洋生态毒理因素的研究奠定良好的基础.Utilizing ecotoxicological and functional genomics technologies,a functional specific cDNA microarray of marine medaka（Oryzias melastigma） was designed and constructed in this study.The microarray contained 180 genes classified into eight functional categories,disruption,xenobiotics detoxification,hypoxia,oxidative stress responses,apoptosis,growth and gametogenesis,sex determination and gonad differentiation,as well as reproductive hormones.Upon exposure of marine medaka adult fishes to normal circumstances or low oxygen tension for 12 weeks,changes in gene expression in the liver and ovary were investigated using a self-made cDNA microarray.The results indicated that nine and six genes expressing the difference were detected in the liver and the ovary,respectively,when the medaka was under low oxygen tension,which clearly indicated that this self-made chip could effectively acquire the gene expression profile.The application of high-quality gene arrays provides a good foundation for developing novel and commercially available products for ecotoxicological risk assessment.Additionally,the results of this study may help elucidate the action mechanisms of toxicants.

关 键 词：海洋生物学 海洋青鳉鱼 生态毒理学 生物芯片 缺氧 

分 类 号：X171[环境科学与工程—环境科学]