谷氨酰胺对延边黄牛卵母细胞成熟及重组胚发育的影响  

Effects of glutamine on the oocyte maturation and development of reorganization embryos in Yanbian cattle

作　　者：韩岩[1] 庄利利[1] 王玉涵[1] 孙武胜[1] 李钟淑[1] 方南洙[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《畜牧与兽医》2010年第5期11-14,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30860184);延边大学211工程三期立项项目(YN-002-2)

摘　　要：为了提高卵母细胞的成熟率及重组胚胎的发育率,在体外成熟液中添加了不同浓度的谷氨酰胺,研究谷氨酰胺对延边黄牛卵母细胞成熟的影响,并观察它对重组胚胎后续发育的影响,从中筛选出最佳的体外培养条件。结果表明,1.0 mmol/L的谷氨酰胺能够显著提高卵母细胞的成熟率,同时也能提高囊胚发育率。说明谷氨酰胺能够提高延边黄牛卵母细胞的体外成熟率,并能促进囊胚发育。To increase the maturation rate of oocytes and the development rate of reorganization embryos,different concentrations of glutamine were added into the in vitro maturation media to study the effects of glutamine on oocyte maturation and development of later embryos in Yanbian cattle,and to determine the best in vitro culture conditions.The results showed that 1.0 mmol/L of glutamine could significantly improve the maturation rate of oocytes,but also improve the development rate of blastocyst.It suggested that glutamine can improve in vitro maturation rate of the oocytes in Yanbian cattle,and promote the development of blastocysts.

关 键 词：延边黄牛 体细胞克隆 体外成熟 重组胚 谷氨酰胺 

分 类 号：S814.8[农业科学—畜牧学]