MicroRNA-134在FMR1基因敲除鼠脑组织中的表达及意义  

Expression and significance of microRNA-134 in mouse brain tissue with FMR1 gene knockout

作　　者：曾志涌 邸伟[2] 肖都[2] 孙逊沙[2] 王玉良[2] 欧阳梅[2] 易咏红 

机构地区：[1]深圳市龙岗区布吉人民医院儿科,518122 [2]广州医学院第二附属医院神经内科,广州医学院神经科学研究所,广州510260

出　　处：《中华神经医学杂志》2010年第8期765-767,共3页Chinese Journal of Neuromedicine

摘　　要：目的 观察脆性X综合征（FXS）模型小鼠不同发育时期脑组织中microRNA-134（miR-134）的表达,明确miR-134的表达特点及脆性X智力低下蛋白（FMRP）缺失是否导致miR-134转录的改变. 方法 应用荧光实时定量PCR检测FVB近交系雄性0 d、4、6周（W）龄FMR1基因敲除型（KO）（KO0d、KO4w、KO6w）和同龄野生型（WT）（WT0d、WT4w、WT6w）小鼠脑组织中miR-134的表达（n=5）. 结果同龄KO与WT小鼠miR-134的转录表达量差异无统计学意义（P＞0.05）;KO6w小鼠脑组织miR-134的转录表达量低于KO0d和KO2w小鼠,WT6w小鼠脑组织miR-134的转录表达量也低于WT0d和WT2w小鼠,差异均有统计学意义（P＜0.05）. 结论 FMRp的缺失并未影响miR-134的转录;miR-134的转录表达量在神经系统发育期保持着较高水平,至成年期则下降,提示其在调控神经系统发育中可能起着重要作用.Objective To observe the expression ofmicroRNA-134 （miR-134） in the mouse brain tissue with FMR1 gene knockout during the different development periods and its expression characteristic, and explore whether the deficiency of fragile X mental retardation protein （FMRP） can induce the changes of miR-134 transcription. Methods FVB strain male mice, including FMR1 gene knockout （KO, n=15） and their wild type （WT, n=15） counterparts were chosen in the experiment. The expressions of miR-134 in the brain tissues of these KO mice that were 0 d, 4 and 6 w old and the age-matched WT mice were detected by qRT-PCR. Results The transcriptional level of miR-134 in the brain tissue of KO mice had no significant difference as compared with that of age-matched WT mice （P＞0.05）. The transcriptional levels of miR-134 in 6-w-old KO and WT mice were significantly decreased as compared with the newbom and 4-w-old same genotype mice （P＜0.05）. Conclusion The absence of FMRP does not influence the transcription of miR-134 and the transcriptional level of miR-134 in the brain tissues maintains a high level during the developmental stage of the nervous system and gradually decreases to a low level after grow-up, demonstrating its important role in regulating the development of nervous system.

关 键 词：脆性X综合征 微RNAS 脆性X智力低下蛋白 

分 类 号：R596.1[医药卫生—内科学]