结核分枝杆菌异烟肼耐药基因katG的检测  

Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis

作　　者：薛欣[1,2] 邱奕 楚雍烈[1] 赵四海[1] 邵明明[1] 曹春霞[1] 

机构地区：[1]西安交通大学医学院病原生物学教研室,陕西西安710061 [2]西安市结核病医院,陕西西安710061

出　　处：《中国病原生物学杂志》2010年第7期496-497,523,共3页Journal of Pathogen Biology

基　　金：西安市科技创新支撑计划项目(No.YF07158)

摘　　要：目的探讨结核分枝杆菌异烟肼(INH)耐药的分子机制,建立快速检测结核分枝杆菌INH耐药性的方法。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测105株结核分枝杆菌临床分离株的katG基因,运用DNA测序和生物信息分析比对进行验证。结果以H37Rv标准株为对照,34株敏感株中5株(14.7%)存在KatG基因异常,105株耐药株中55株(52.4%)存在KatG基因异常。结论结核分枝杆菌对INH耐药与KatG基因突变有关,PCR-RFLP技术可快速、准确地检测结核分枝杆菌对INH的耐药性。Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid （INH） and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 （14.7%） drug-susceptible isolates and 55 of 105 （52.4%） INH-resistant isolates had a katG gene mutation according to PCR-RFLP.Conclusion Drug resistance of M.tuberculosis to INH is related to katG gene mutation,PCR-RFLP might serve as a method of rapidly detecting the INH resistance of M.tuberculosis.

关 键 词：分枝杆菌 结核 耐药 异烟肼 基因突变 限制性片段长度多态性 

分 类 号：R378.911[医药卫生—病原生物学]