抗生长分化因子-9抗体与化疗所致大鼠卵巢损伤的关系的研究  

The relationship between Anti-Growth differentiation faotor-9 and chemotherapy-induced ovarian damage in rats

作　　者：潘秀芹 肖苑玲[2] 王永红 

机构地区：[1]广东省清新县人民医院妇产科,广东清新511800 [2]南方医科大学珠江医院妇产科,广东广州510280

出　　处：《中国当代医药》2010年第22期15-17,共3页China Modern Medicine

摘　　要：目的:探讨抗生长分化因子-9抗体(Anti-GDF-9)对化疗所致大鼠卵巢损伤是否具有防治作用。方法:将96个大鼠卵巢分为6组:①正常对照组12个;②磷酰胺氮芥(PM)组12个;③生理盐水组12个;④同时加抗体组20个;⑤先加抗体组20个;⑥后加抗体组20个。加入抗体的3组有12个卵巢培养8 d,8个卵巢培养14 d,其余3组均培养8 d,隔天换液;按上述要求取出标本,称湿重,HE染色,计数卵泡总数。结果:培养8 d,正常对照组和同时加抗体组的卵泡总数比较,差异无统计学意义(P>0.05),其余各组两两比较卵泡总数差异均有统计学意义(P<0.05),其中先加抗体组多于正常对照组;培养14 d,加入抗体的3组两两比较卵泡总数差异均有统计学意义,先加抗体组卵泡总数最多。结论:体外实验,在加入PM前或同时加入Anti-GDF-9可能会减轻PM对大鼠卵巢的损伤。Objective： To study the effect of Anti-Growth differentiation faotor-9（Anti-GDF-9） against glyciphosphoramide（PM） induced ovarian damage in female rats.Methods： Ninety-six ovaries（forty-eight Wistar rats）were randomly divided into six groups,such as normal control group（n=12）,PM group（n=12）,saline group（n=12）,PM＋ Anti-GDF-9 groups（with Anti-GDF-9 administerd simultaneously,before and after PM separately for 24 h,20 each）.To culture the ovarian tissue in 24 hole culture plates,one ovary each hole,for 8 days or 14 days（PM＋ Anti-GDF-9 groups,8 ovaries each group）,replaced culture medium（no PM） every two days for all ovaries.Weighting,HE staining and counting the number of follicles of each phase.Results： There showed significant difference in the number of follices between every two groups（P〈0.05） except between the normal control group and the group which administerd Anti-GDF-9 simultaneously with PM at day 8 after culture,and the number of follicles of the group which administerd Anti-GDF-9 before PM was more than the normal control group.There showed significant difference in the number of follices between every two groups of the PM＋Anti-GDF-9 groups at day 14 after cultured,and the group which administerd Anti-GDF-9 before PM had the largest number of follicles.Conclusion： Anti-GDF-9 administerd before PM for 24 h and simultaneously with PM can diminished PM induced ovarian damage in ovaries in vitro.

关 键 词：抗生长分化因子-9抗体 磷酰胺氮芥 化疗 大鼠 卵巢损伤 

分 类 号：R-332[医药卫生]