玉米AGPase胞质型小亚基ZmSSUI基因的克隆及在籽粒发育过程中的表达  

Cloning of a Cytosolic Small Subunit of AGPase and its Transcripts in the Developing Kernels of Maize(Zea mays)

作　　者：沈丙权[1] 郑贝贝[1] 彭慧芳[1] 郭天财[1] 康国章[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,郑州450002

出　　处：《西北农业学报》2010年第7期61-65,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(30871472);国家转基因专项子课题(082102140020-003和2009ZX08002-21B);作物生物学国家重点实验室开放课题(2009KF01)

摘　　要：利用RT-PCR方法从普通玉米浚单20中克隆出淀粉合成关键酶-腺苷二磷酸葡萄糖焦磷酸化酶（ADP-Glucose Pyrophosphorylase,AGPase）胞质型小亚基（cytosolic small subunit,SSUI）基因的cDNA序列,命名为ZmSSUI（GenBank登录号：GU550073）。序列分析表明,该cDNA序列长度1554 bp,包含一个完整的开放阅读框（2~1 429 bp）,长度为1 428 bp,编码476个氨基酸,克隆基因编码的氨基酸序列与其他植物SSUI氨基酸序列有较高同源性。半定量RT-PCR分析表明,在籽粒发育过程中,ZmSSUI基因的表达呈单峰状变化,与已报道的AGPase酶活性及淀粉积累速率趋势基本一致,推测其在玉米淀粉合成中发挥着重要作用。A cDNA sequence encoding a cytosolic small subunit gene of AGPase was isolated from maize（Zea mays） by RT-PCR and designated as ZmSSU I（GenBank Accession No.GU550073）.Its length was 1 554 bp,containing an open reading frame（ORF） of 1 428 bp encoding a putative protein of 476 amino acids.Sequence alignment indicated that the putative amino acid sequence of ZmSSU I shared high identities 96%,94%,91% and 89% to Zea mays（AAN39299）,Sorghum bicolor（ABK97531）,Oryza sativa（ACJ86322） and Triticum aestivum（AAF61173）,respectively.The transcription levels of ZmSSU I in maize kernels were analyzed with semi-quantitative RT-PCR method.Results showed that the expression of ZmSSU I in developing kernels had a single-peak curve occurring 30 d after pollination.The results showed that ZmSSU I played an important role in maize starch biosynthesis.

关 键 词：玉米 AGPASE 胞质型小亚基 淀粉 

分 类 号：S513[农业科学—作物学]