识别肺癌上调表达的膜蛋白单抗抑制肺癌生长  

Growth of lung cancer can be inhibited by monoclonal antibody recognizing lung cancer membrane protein expressed highly

作　　者：杨薇薇[1] 遇珑[2] 孙力超[2] 孙立新[2] 李江伟[1] 杨治华[2] 冉宇靓[2] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室细胞及分子生物学研究室,北京100021

出　　处：《中国卫生检验杂志》2010年第7期1586-1589,1592,共5页Chinese Journal of Health Laboratory Technology

基　　金：国家重点基础研究发展计划(973)项目(2009CB521804);国家科技重大专项课题(2009ZX09401-005)

摘　　要：目的:分析研究抗肺癌24H7单抗的体内外功能,并初步鉴定其识别的抗原蛋白,为肺癌靶向治疗提供候选抗体药物及靶标。方法:采用活细胞免疫荧光及流式细胞荧光检测单抗与多种肺癌细胞的反应;应用CCK-8法和裸鼠体内成瘤实验鉴定单抗对肺癌细胞体外增殖及体内肿瘤生长的影响;同时采用免疫组化检测该单抗识别抗原在肿瘤及癌旁正常组织中的表达差异;免疫亲和层析纯化抗原。结果:活细胞免疫荧光及流式荧光结果显示24H7单抗识别抗原在12种不同肺癌细胞系膜上均有阳性表达;体外增殖及动物体内治疗实验显示该单抗在体外能抑制肺癌细胞的增殖,并在体内显著抑制肿瘤的生长,其抑制率达73.74%(P<0.01);免疫组化结果表明该单抗的靶抗原在肺腺癌组织中的表达较癌旁正常显著上调;免疫亲和层析显示靶抗原的分子量约为170 KD。结论:抗肺癌单抗24H7能够在体内外抑制肺癌的生长,其靶抗原可表达于肺癌细胞的膜上,且在肺腺癌组织上调表达,可能是一个肺腺癌靶向治疗的新靶标。Objective：Study the function in vitro and in vivo of 24H7 monoclonal antibody targeting lung cancer and identify the antigen,to provide a candidate antibody drug and molecule target for the anti-lung cancer therapy.Methods：Binding with viable lung cancer cells of 24H7 monoclonal antibody were studied by immunofluorescence and flow cytometry of viable cells;the inhibition of lung cancer cells and implanted tumor of 24H7 antibody were studied by cell proliferation assay and tumor treatment experiment in vivo;the expressional difference of antigen in lung cancer tissues and corresponding normal tissues of 24H7 antibody was detected by immunohistochemistry;the antigen of 24H7 antibody were purified by immunoaffinity chromatography.Results：The 24H7 antibody could recognize cell membrane protein of 12 lung cancer cell lines;obviously inhibit lung cancer cells proliferation and tumor growth in vivo by 73.74%（P0.01）.Immunohistochemistry showed higher expression on the adenocarcinoma of lung cancer tissues.The antigen of 24H7 antibody which molecular weight about 170 KD gained by immunoaffinity chromatography.Conclusion：24H7 monoclonal antibody can surpress lung cancer cells proliferation in vitro and tumor growth in vivo;the antigen of 24H7 expressed on cell membrane and highly on the adenocarcinoma of lung cancer tissues,it might become a candidate molecular for targeted adenocarcinoma of lung cancer treatment.

关 键 词：肺癌 膜蛋白 功能性单克隆抗体 抗原 靶标 

分 类 号：R734.2[医药卫生—肿瘤]