α1,2-岩藻糖转移酶基因转染对卵巢癌细胞RMG-I裸鼠体内致瘤性及血管生成因子表达的影响  

Effect of α1,2-fucosyltransferase Gene Transfection on Tumorigenicity and Angiogenic Growth Factor Expression in Ovarian Carcinoma-derived RMG-I Cells of Nude Mice

作　　者：李妍[1,2] 李飞飞[3] 林蓓[1] 郝莹莹[1] 刘娟娟[1] 刘晴[1] 朱连成[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004 [2]沈阳市妇女儿童保健中心妇产科,沈阳110036 [3]山东大学附属省立医院妇产科,济南250021

出　　处：《中国医科大学学报》2010年第7期505-507,517,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30170980;30571958;30872757);教育部博士点基金资助项目(20070159023)

摘　　要：目的比较α1,2岩藻糖转移酶(α1,2-FT)基因转染前后卵巢癌细胞株RMG-I的裸鼠体内致瘤性及移植瘤组织中血管内皮生长因子(VEGF)、转化生长因子-β(1TGF-β1)及碱性成纤维生长因子(b-FGF)表达的变化。方法我们在前期工作中利用基因转染技术将α1,2-FT基因转入人卵巢癌细胞株RMG-I,建立了α1,2-FT及Lewisy高表达细胞株RMG-I-H。本实验将转染前后细胞种植裸鼠皮下建立人卵巢癌裸鼠移植瘤模型,观察两组肿瘤生长情况。5周后处死动物,测量移植瘤质量,免疫组织化学方法检测肿瘤组织VEGF、TGF-β1及b-FGF的表达。结果两组裸鼠均有皮下荷瘤形成,但转染组的成瘤时间(5.2±0.8d)早于未转染组(8.8±1.3d),且转染组的瘤质量和体积与未转染组相比亦明显增加(P<0.05)。转染组裸鼠移植瘤组织VEGF、TGF-β1及b-FGF的表达均明显高于未转染组(P均<0.05)。结论α1,2-FT基因转染能增强卵巢癌细胞RMG-I的体内致瘤性,上调裸鼠移植瘤组织VEGF、TGF-β1及b-FGF的表达。提示α1,2-FT及Lewisy抗原参与血管生成,其高表达在促进血管生成中可能发挥重要作用。Objective To compare the changes in tumorigenicity and expressions of vascular endothelial growth factor（VEGF）,transforming growth factor-β1（TGF-β1）,and basic fibroblast growth factor（b-FGF）in ovarian carcinoma-derived RMG-I cells of nude mice before and after transfection of α1,2-fucosyltransferase（α1,2-FT）gene.Methods Previously,we transfected the ovarian cancer cell line RMG-I with α1,2-FT gene using gene transfection technology and established cell line RMG-I-H with high expressions of α1,2-FT and Lewis y antigen.In this study,nude mice were subcutaneously injected with RMG-I-H and RMG-I cells.After 5 weeks,the mice were killed,and the weight of formed tumors was measured.Tumorigenicity was observed,and the expressions of VEGF,TGF-β1,and b-FGF in tumor tissue were detected by immunohistochemistry.Results Subcutaneous tumor was formed in nude mice in both transfected and non-transfected groups.The time of tumor formation in transfected group was earlier than that in non-transfected group（5.2±0.8 vs.8.8±1.3 d）,and the tumor weight and volume in transfected group were significantly increased compared with non-transfected group（P〈0.05）.The expressions of VEGF,TGF-β1,and b-FGF in transplanted tumor tissue was significantly higher in transfected group than in non-transfected group（P〈0.05）.Conclusion α1,2-FT gene transfection can enhance the tumorigenicity of RMG-I cells in vivo and up-regulate the expressions of VEGF,TGF-β1,and b-FGF in transplanted tumor tissue.These results suggest that α1,2-FT and Lewis y antigen may be involved in angiogenesis,and the high expressions of α1,2-FT and Lewis y antigen may play important roles in the promotion of angiogenesis.

关 键 词：卵巢癌 Lewisy抗原 血管生成 裸鼠 VEGF TGF-β1 B-FGF 

分 类 号：R737.31[医药卫生—肿瘤]