联合应用全反式维甲酸与单纯疱疹病毒腺苷激酶基因对髓母细胞瘤的协同杀伤作用研究  

Synergistic Effect of All-trans Retinoic Acid and Herpes Simplex Virus Thymidine Kinase Gene on Medulloblastoma

作　　者：蒲珂[1] 李少一[1] 马丽[2] 高芸[3] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,沈阳110004 [2]上海交通大学医学院附属第九人民医院口腔医学院,上海200011 [3]中国医科大学附属第一医院内分泌研究所,沈阳110001

出　　处：《中国医科大学学报》2010年第8期596-598,602,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30772246;30700861);辽宁省博士启动基金资助项目(20071046)

摘　　要：目的评估全反式维甲酸(ATRA)联合单纯疱疹病毒腺苷激酶(HSV-tk)基因对髓母细胞瘤细胞系Daoy的杀伤作用。方法观察不同浓度ATRA对Daoy细胞生长的抑制作用;HSV-tk基因稳定转染人的髓母细胞瘤细胞系Daoy,获得Daoy-tk细胞;Daoy-tk细胞与Daoy细胞按不同比例混合,给予不同浓度的更昔洛韦(GCV)和一定浓度的ATRA,7d后应用噻唑蓝(MTT)法测定细胞生存率。结果 ATRA在0.5～1μmol/L时即可对Daoy细胞生长产生抑制作用;Daoy-tk与Daoy细胞比例达到1:4时仍有明显的抗肿瘤作用,联合应用1μmol/L的ATRA后能显著提高HSV-tk基因对Daoy细胞的杀伤率。结论 ATRA与HSV-tk/GCV系统联合应用,能增强对人的髓母细胞瘤的杀伤作用。Objective To evaluate the synergistic lethal effect of all-trans retinoic acid and herpes simplex virus-thymidine kinase gene on medulloblastoma（MB）.Methods MB cell line Daoy cells were exposed to different concentrations of ATRA,to study the inhibitory effect of ATRA on Daoy cells;Co-cultured Daoy-tk and Daoy cells at various ratios,treated with different concentrations of GCV and a certain concentration of ATRA.The viability of the cells were measured on day 7 with MTT assay.Results ATRA could inhibit the grow of Daoy cells at the concentration 0.5 to 1μmol/L.The Daoy-tk cells had an evident anti-tumor effect when Daoy-tk：Daoy at the ratio as low as 1：4,and the antitumor effect was stronger with 1μmol/L ATRA.Conclusion Combination of ATRA and HSV-tk/GCV system have a synergistic killing effect on medulloblastoma.

关 键 词：全反式维甲酸 单纯疱疹病毒腺苷激酶 更昔洛韦 髓母细胞瘤 

分 类 号：R739.41[医药卫生—肿瘤]