气相色谱法测定白苏子与紫苏子中亚油酸和α-亚麻酸的含量  

Determination of Linoleic Acid andα-linolenate Acid in Seeds of Perilla frutescens(L.)Britt.by GC

作　　者：芦雅丽[1] 赵建邦[2] 宋平顺[2] 

机构地区：[1]兰州大学药学院,兰州730000 [2]甘肃省药品检验所

出　　处：《中国药师》2010年第8期1087-1089,共3页China Pharmacist

基　　金：甘肃省科学技术攻关计划项目(编号:GS035-A43-348-03)

摘　　要：目的:建立苏子中亚油酸和α-亚麻酸的含量测定方法;比较白苏子与紫苏子两种品系的含量差异。方法:采用气相色谱法。ZB-WAX柱(30 m×0.32 mm,0.5μm)。流动相为氮气(纯度99.999%),流速1.0 ml·min^(-1),分流比为25:1。柱温:190℃。进样口温度为250℃。FID检测器温度250℃;流速:空气450 ml·min^(-1),氢气45 ml·min^(-1)。结果:亚油酸在0.351～17.535μg范围内呈良好的线性关系,r=0.999 8(n=6),平均回收率为103.62%,RSD为1.0%;α-亚麻酸在0.270～13.514μg范围内呈良好的线性关系,r=0.9999(n=6),平均回收率为100.88%,RSD为1.3%;结论:测定方法准确、重复性好,可用于苏子中亚油酸与α-亚麻酸的定量测定。Objective： To develop a GC method for determination of α-linolenate acid and linoleic acid, and compare the content in two kinds of seeds of Perillafrutescens （L.） Britf. Method： The samples were separated on an ZB-WAX （30 m ×0. 32 mm,0. 5 μm） column. Purity of nitrogen was （99.999%）. Flow rate was 1.0 ml. min^-1 , split ratio was 25： 1. Column temperature was 190℃. Injection temperature was 250℃. The detective temperature of FID was 250℃, the flow rate of air and hydrogen was 450 ml·min^-1 and 45 ml·min^-1 respectively. Result： The linear range of linoleic acid was 0. 351-17. 535 μg（r =0. 999 8 ,n = 6） ,the average recovery was 103.62% ,RSD was 1.0% ;the linear range of α-linolenate acid was 0. 270-13. 514 μg （r =0. 999 9 ,n =6） ,the average recovery was 100. 88% ,RSD was 1.3%. Conclusion： The method is accurate and reliable, and it provides a method basis for Perilla frutescens （L.） Britt.

关 键 词：苏子 Α-亚麻酸 亚油酸 气相色谱 含量测定 

分 类 号：R927.2[医药卫生—药学]