芹菜素对3T3-L1前脂肪细胞增殖及分化作用研究  

Effect of Apigenin on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

作　　者：于华强[1] 籍保平[1] 柳嘉[1] 周峰[1] 高丰衣[1] 苏春元[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2010年第15期260-263,共4页Food Science

摘　　要：目的:研究芹菜素(apigenin,AP)对3T3-L1前脂肪细胞增殖及分化的影响。方法:采用3T3-L1前脂肪细胞,利用MTT比色分析法检测不同质量浓度芹菜素对3T3-L1前脂肪细胞增殖的影响;采用传统的鸡尾酒诱导剂诱导分化3T3-L1前脂肪细胞,利用油红O染色检测,通过比色分析法检测不同质量浓度芹菜素对3T3-L1前脂肪细胞分化的影响。结果:30、50μg/mL芹菜素相对于空白组和阳性对照组能够显著抑制3T3-L1前脂肪细胞增殖和分化,提示其可能具有预防肥胖的作用。Objective：To investigate the effect of apigenin on proliferation and differentiation of 3T3-L1 preadipocytes.Methods：3T3-L1 preadipocytes were used as target cells to explore the effect of apigenin on their proliferation measured by MTT assay.Besides,cell differentiation using differentiation cocktail,oil red O staining and spectrometric determination were carried out for investigating the effect of apigenin on the differentiation of 3T3-L1 preadipocytes.Results：Apigenin at 30μg/mL and 50 μg/mL concentrations could inhibit the proliferation and differentiation of 3T3-L1 preadipocytes in comparison with the blank group and the positive control group（established using Dulbecco smodified Eagle smedium containing 0.5 mmol/L IBXM,1μmol/L dexamethasone and 10μmol/L insulin）.Conclusion：Apigenin appear to have potential for the prevention of obesity.

关 键 词：芹菜素 3T3-L1 增殖 分化 肥胖 

分 类 号：Q946.8[生物学—植物学]