一株光合细菌菌株RPD-1的生物学特性及其在菲律宾蛤仔育苗中的应用  

Characterization of photosynthesis bacteria strain RPD-1 and its application in the breeding of Ruditapes philippinarum

作　　者：王芳[1] 李筠[2] 盛军[1] 孙谧[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国海洋大学,山东青岛266003

出　　处：《海洋科学》2010年第7期1-7,共7页Marine Sciences

基　　金：中央级科研院所基本科研项目(2007-gy-04)

摘　　要：RPD-1菌株分离自青岛栈桥近岸海水,根据其生理生化特征鉴定为紫色非硫菌科红假单胞菌属(Rhodopseudomonas)。菌株RPD-1适盐范围较广,在NaCl含量为0～8%的培养基中均可生长,在NaCl为3%的培养基中生长最快;在初始pH为6.0～9.0的培养基中均可生长,pH为7.5时生长最快;不同光源的光照条件下,生长速度不同,白炽灯光照下,生长速度快,日光灯照下,生长速度慢;RPD-1菌株蛋白质含量为58.5%,含各种必需氨基酸。菲律宾蛤仔(Ruditapes philippinarum)育苗实验结果表明,RPD-1菌株能提高菲律宾蛤仔幼虫的存活率和变态率;水体氨态氮测定结果表明,RPD-1菌株能显著降低养殖水体中的氨氮。The Photosynthesis Bacteria strain RPD-1 was isolated from seawater near the Qingdao Pier. According to physiological and biochemical characteristics, the RPD-1 strain was identified as Rhodopseudomonas sp. The RPD-1 strain growed well in the mediums containing 0～8% NaCl and the optimum salinity is 3%. Medium pH rangeing from 6.0 to 9.0 was appropriate for the RPD-1 strain, with the optimum pH being 7.5. The RPD-1 strain growed faster under the light of filament lamp than that of flurescent lamp. The protein content of RPD-1 strain was 58.5%. There are various necessary amino acids. The ratios of livability and metamorphosis of Ruditapes philippinarum were increased by adding the RPD-1 strain of suitable concentrations to the breeding water. When the RPD-1 strain of suitable concentration was added to the breeding water, the contents of nitrogen in the state of ammonia decreased significantly.

关 键 词：红假单胞菌属(Rhodopseudomonas) 菲律宾蛤仔(Ruditapes philippinarum) 存活率 变态率 

分 类 号：S931[农业科学—渔业资源]