用碳糊修饰电极测定氨基酸的伏安法研究 Ⅰ.色氨酸和酪氨酸在聚酰胺修饰碳糊电极上的电化学行为  

Voltammetric Determination of Amino Acidsby Modified Carbon Paste ElectrodesⅠ. Study on the Electrochemical Behaviour of Tryptophan and Tyrosine on Polyamide _ modified Carbon Paste Electrode

作　　者：邹永德[1] 王进[1] 莫金垣[1] 张润建[1] 

机构地区：[1]中山大学化学系

出　　处：《分析测试学报》1999年第2期25-28,共4页Journal of Instrumental Analysis

摘　　要：用含１０％聚酰胺的修饰碳糊电极研究了测定色氨酸和酪氨酸的伏安法，发现在０．２ｍｏｌ／Ｌ盐酸－醋酸钠底液中色氨酸和酪氨酸有良好的氧化峰响应。结合２．５次微分技术，两种氨基酸的检测浓度线性范围良好，色氨酸和酪氨酸的相对标准偏差分别为５．２％和７．０％，检测限分别为０．０２４μｍｏｌ／Ｌ和０．０３４μｍｏｌ／Ｌ。检测特性比文献报道有较大的改善。文中对电极过程进行了讨论。The determination of tryptophan and tyrosine has been studied by a polyamide_modified carbon paste electrode. There are good voltammetric responses for those amino acids in supporting electrolyte of 0.2 mol/L HCl-CH3COONa(pH=3.0). Combining 2.5th order differential technique, the method has a good linearity for both amino acids. A relative standard deviation of 5.2% for tryptophan and of 7.0% for tyrosine were obtained, detection limit of 0.024 μmol/L for tryptophan and of 0.034 μmol/L for tyrosine were achieved, respectively. The electrode mechanism was investigated.

关 键 词：色氨酸 酪氨酸 碳糊电极 伏安法 测定 氨基酸 

分 类 号：Q517.03[生物学—生物化学] O629.711[理学—有机化学]