响应曲面分析法对青藤碱超临界CO2流体萃取条件的优化  

Optimal parameters with response surface methodology for supercritical CO_2 extraction of Sinomenine

作　　者：冯自立[1] 陈德经[2] 季晓晖[3] 马娜[4] 

机构地区：[1]陕西理工学院生物科学与工程学院 [2]陕西省资源生物重点实验室,陕西汉中723001 [3]陕西理工学院化学与环境科学学院 [4]陕西理工学院物理系

出　　处：《计算机与应用化学》2010年第7期928-932,共5页Computers and Applied Chemistry

基　　金：陕西省教育厅重点实验室资助项目(08JK245)

摘　　要：以青风藤药材为原料,在单因素实验研究的基础上,采用Box-Behnken响应曲面分析法对影响青风藤药材中青藤碱超临界CO_2萃取得率的关键因素:萃取时间、压力、温度进行了优化,旨在为青藤碱新型、绿色提取分离方法作探讨。根据统计模型进行了工艺参数的优选,以得率为指标,优化后所得青藤碱超临界CO_2萃取工艺条件为:萃取压力为27.63 MPa,萃取温度为50.68℃,萃取时间为120.24 min,该条件下得率为1.372%。验证试验证实了该方程的预测值与试验值之间具有较好的拟合度。The response surface methodology based on a three-level three-factor Box-Behnken experimental design was used to optimize the Sinomenine extraction with supercritical carbon dioxide（SC-CO2） with root of Caulis Sinomenii as materials.The critical factors selected with previous single-factor tests were pressure,temperature and time.The adequacy of the model equation for predicting the optimum response values was verified effectively by the validation. Effects of pressure,temperature and time on extraction ratio were investigated.The optimized technology parameters belower than benzene extraction were pressure of 27.63 MPa,temperature of 50.68℃,time of 120.24 min,and under these conditions the extraction ratio of Sinomenine was up to 1.372%.The error shows that the regressive equation fits the extraction process perfectly.

关 键 词：青藤碱 得率 超临界CO2萃取 响应面分析 BOX-BEHNKEN设计 

分 类 号：O641[理学—物理化学] TQ46[理学—化学]