多不饱和脂肪酸对多巴胺能神经元保护作用  

Effect of n-3 PUFAs in LPS-induced substantia nigra dopaminergic neurons injury

作　　者：刁欢[1] 王枫[1] 王晓慧[1] 骆文静[1] 于芳[1] 侯祥红[1] 蔡同建[1] 张建彬[1] 季爱玲[1] 

机构地区：[1]第四军医大学军事预防医学系营养与食品卫生学教研室,陕西西安710032

出　　处：《中国公共卫生》2010年第8期1075-1076,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30700667)

摘　　要：目的研究饮食补充n-3多不饱和脂肪酸(PUFA)对脂多糖(LPS)诱导的多巴胺神经元损伤的保护作用。方法将雄性SD大鼠随机分为对照组,LPS组,LPS+30%,60%鱼油组,鱼油组基础饲料中添加30%和60%鱼油;黑质内注射LPS 5μg/只,对照组注射生理盐水5μL/只,14 d后,观察大鼠旋转行为及检测黑质酪氨酸羟化酶(TH)含量。结果对照组无明显行为异常,LPS组大鼠旋转圈数(114±36)明显大于30%鱼油组(42±22)和60%鱼油组(18±13)(P<0.05);与对照组比较,LPS组TH阳性细胞数明显减少(P<0.05),30%,60%鱼油组与对照组比较TH阳性细胞数差异无统计学意义。结论通过膳食补充n-3 PUFAs(鱼油),对多巴胺能神经元有一定保护作用。Objective To investigate the protective effect of dietary intake of n-3 polyunsaturated fatty acid（PUFA） on lipopolysaccharide（LPS）-induced dopamine neuron injury.Methods The male SD rats were randomly divided into normal saline（NS） control group,LPS group,LPS＋30% fish oil group,and LPS＋60% fish oil group.The skim-based feed of two fish oil groups was added with 30% and 60% of fish oil.The rats were administered with LPS by stereotaxic injection at the dose of 5 μg / rat.The NS group was injected with NS 5 μl / rat.Fouteen days after the injections,the rotational behavior of the rats was observed and immunofluorescence method was adopted to detect the change of rate-limiting enzyme tyrosine hydroxylase（TH） in the dopamine substantia nigra neurons.Results The rats in NS control group showed no obvious abnormal behavior.The numbers of turning around of the rats in LPS group were obviously larger than that of the rats in two diet groups（P0.05）.Compared with the NS control group,the number of TH-positive cells in LPS group was significantly reduced（P0.05）.But compared with the NS group,the number of TH-positive cells in the fish oil groups showed no significant difference.Conclusion Dietary supplements of n-3 PUFAs or fish oil have protective effect on dopaminergic neurons.

关 键 词：n-3多不饱和脂肪酸(n-3 PUFAs) 脂多糖 多巴胺神经元 

分 类 号：R151.3[医药卫生—营养与食品卫生学]