液相芯片技术在HPV血清检测中的初步应用  

Primary Application of Liquid Chip on HPV Serology Detection

作　　者：姚丽[1] 李艳红 张伟[1] 王姝妹[1] 巩丽[1] 朱少君[1] 韩秀娟[1] 兰淼[1] 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038 [2]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《现代生物医学进展》2010年第14期2632-2634,共3页Progress in Modern Biomedicine

摘　　要：目的:建立一种高效的HPV病毒血清学检测方法。方法:重组质粒表达HPV16/18E6和E7蛋白,同微球偶联后作为抗原,应用Luminex系统检测宫颈癌患者血清中的相应抗体。结果:57.4%宫颈癌患者血清至少检测到HPV16E6、E7和HPV18E6、E7蛋白抗体中1种,36.1%的血清检测到HPV16E6和E7蛋白抗体中1种,31.9%的血清检测到HPV18E6和E7蛋白抗体中1种。结论:基于GST重组HPV蛋白的液相芯片技术可进一步应用于患者血清HPV抗体的检测中。Objective：To develop a new simultaneous method to detect the human papilloma virus （HPV） serology.Methods：Distinct bead sets, each carrying one sort of HPV protein were mixed, incubated with serum, which were detected by Luminex technology.Results 57.4% of the serum from patients with cervical carcinomas reacted positively in at least one of the four HPV antigens, 36.1% reacted positively in HPV-16 E6 and/or E7 and 31.9% was positive at least one of the two HPV-18 proteins.Conclusions：This new method could be used in HPV serology detection.

关 键 词：液相芯片技术 人乳头状瘤病毒 血清检测 

分 类 号：R362[医药卫生—病理学] R446[医药卫生—基础医学]