信号转导和转录激活因子3及其靶基因c-myc在喉鳞状细胞癌中的表达与意义  

Expression and significance of signal transducer and activator of transcription 3 and c-myc in laryngeal squamous cell carcinoma

作　　者：刘亭彦[1] 彭华光[2] 吴正虎[1] 崔彩霞[1] 

机构地区：[1]杭州师范大学附属医院耳鼻咽喉-头颈外科,杭州310015 [2]杭州师范大学附属医院口腔科,杭州310015

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第14期648-651,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:检测喉鳞状细胞癌中信号转导和转录激活因子3(STAT3)基因及其靶基因c-myc的表达,探讨其临床意义及相关性。方法:采用原位杂交技术和免疫组织化学技术,在mRNA和蛋白2个水平检测STAT3和c-myc基因在56例喉鳞状细胞癌和30例喉全(或近全)切除者癌旁(>2.0cm)正常黏膜中的表达,应用显微图像分析系统测定阳性表达的平均吸收度值,并进行统计分析。结果:在喉鳞状细胞癌组织中存在STAT3和c-myc基因的mRNA和蛋白高表达;STAT3和c-myc基因的mRNA表达与喉鳞状细胞癌的临床分期、分化程度和淋巴结转移有关(P<0.05或P<0.01);STAT3和c-myc基因的蛋白表达与喉鳞状细胞癌的临床分期和淋巴结转移有关(P<0.05或P<0.01);在喉鳞状细胞癌组织中STAT3和c-myc基因的高表达呈正相关(r=0.6224、0.7012,均P<0.01)。结论:STAT3基因及其靶基因c-myc的表达与喉鳞状细胞癌的发生、发展和转移等生物学行为有关,两者的表达呈正相关。Objective：To investigate the expression and clinical siginificance of signal transducer and activator of transcription 3（STAT3）and its target gene c-myc in laryngeal squamous cell carcinoma（LSCC）,and the relationship between the two genes.Method：Formalin-fixed and paraffin-embedded tissues,which came from 56 cases of LSCC and 30 samples of normal mucosas from 30 patients with total or subtotal laryngectomy over 2.0 cm away from tumor margin,were detected for the expression of STAT3,c-myc by in situ hybridization and SP immunohistochemistry.Micro-image analysis system was used to determine the optical density,and the result was analyzed statistically.Result：There is overexpression of STAT3,c-myc mRNA and protein in LSCC.The expression of STAT3 and c-myc mRNA in LSCC was associated with clinical stage,differentiation grade and lymph nodal metastases（P〈0.05 or 0.01）.The expression of STAT3 and c-myc protein in LSCC was associated with clinical stage and lymph nodal metastases（P〈0.05 or 0.01）.There was a positive correlation between the expression of STAT3 and c-myc genes.mRNA and protein,The correlation coefficient（r）was 0.6224（P〈0.01）for the mRNA expression and 0.7012（P〈0.01）for the protein expression.Conclusion：The expression of STAT3 and c-myc may play an important role in the tumorigenesis,metastases and poor prognosis of LSCC.There was a positive correlation between the overexpression of STAT3 and c-myc genes in LSCC.

关 键 词：喉肿瘤 鳞状细胞癌 信号转导和转录激活因子3 C-MYC 原位杂交 

分 类 号：R739.6[医药卫生—肿瘤]