大鼠脑缺血模型的改进和脑缺血再灌流损伤诱发细胞凋亡的研究  

Improving of the model of cerebral ischemia in the rats and apoptosis induction in reperfusion after cerebral ischemia

作　　者：叶建锋[1,2,3] 李涛平[1,2,3] 舒斯云 高宁[1,2,3] 

机构地区：[1]广州第一军医大学基础部 [2]第一军医大学南方医院 [3]第一军医大学珠江医院

出　　处：《中国急救医学》1999年第4期197-199,共3页Chinese Journal of Critical Care Medicine

摘　　要：目的改进动脉内置线阻断法建立的大鼠大脑中动脉（ＭＣＡ）局灶性脑缺血模型，并探讨脑缺血诱发细胞凋亡的时效和量效关系。方法以顶端细，后逐渐增粗的涂抹硅橡胶改进栓子，ＴＴＣ染色鉴定缺血效果；ＴＵＮＥＬ－ＡＰ法和ＨＥ染色观察凋亡发生和形态学改变。结果ＴＴＣ染色证实了大脑缺血灶的的稳定出现；用ＴＵＮＥＬ法发现，缺血３０ｍｉｎ再灌流６ｈ后，凋亡阳性细胞即明显增多，４８ｈ再灌流后阳性细胞数最多；缺血５ｍｉｎ再灌流４８ｈ缺血脑区的凋亡细胞主要位于纹状体，１５ｍｉｎ缺血组皮层也开始散见阳性细胞；随缺血时间延长，凋亡细胞主要出现在缺血区周边。结论脑缺血可选择性诱发神经细胞凋亡。Objective To improve the model of intraluminal vascular occlusion of the middle cerebral artery in rats and assess the effects of focal ischemia on apoptosis induction.Methods The embolus was smeared thin in top and than gradually thicker in the end with silicon-rubber.The TTC staining was employed to appraise the ischemic effect.The TUNEL labeling and HE staining were used to detect apoptotic cells and morphologic changes in ischemic brain.Results The occlusion of ipsilateral MCA produced well-defined lesions that could be visualized with TTC staining.With the TUNEL labeling,apoptotic cells was increased as early as 6 hours after 30-min MCA occlusion and peaked at 48-hour reperfusion.Apoptotic cell increased in striatum after 5-min ischemia and 48-hour reperfusion,and scattered in the cortex after 15-min ischemia.With the time of ischemia prolonged,groups of apoptotic cells were primarily localized to the inner boundary zone of the infarct.Conclusion Neurocyte apoptosis can be selectivity induced in ischemic brain. [

关 键 词：脑缺血 细胞凋亡 再灌注损伤 实验研究 

分 类 号：R743.31[医药卫生—神经病学与精神病学]