半边旗中二萜类化合物6F对肝癌细胞SPC-A-1细胞周期及细胞DNA、RNA和蛋白质合成的影响  

EEThe effect of compoud 6F isolated from Pteris Semipinnata L on cell cycle and synthesis of DNA, RNA and protein of lung adenocarcinoma cell 

作　　者：李金华[1] 梁念慈[1] 莫丽儿[1] 何承伟[1] 张晓[1] 

机构地区：[1]广东医学院医用生化研究所

出　　处：《中国药理学通报》1999年第1期49-51,共3页Chinese Pharmacological Bulletin

基　　金：广东省科委重点科技攻关项目

摘　　要：目的研究半边旗中二萜类有效成分之一６Ｆ对肺癌细胞细胞周期及细胞中ＤＮＡ、ＲＮＡ和蛋白质合成的影响。方法采用体外培养的人癌细胞株——肺腺癌上皮细胞ＳＰＣ－Ａ－１作实验对象，以噻唑蓝（ＭＴＴ）法测定细胞存活率；用细胞光度术（ＦＣＭ）测定６Ｆ对ＳＰＣ－Ａ－１细胞周期的影响［３Ｈ］－ＴｄＲ、［３Ｈ］－ＵＴＲ和［３Ｈ］－Ｌｅｕ参入的方法观察６Ｆ对ＳＰＣ－Ａ－１细胞ＤＮＡ、ＲＮＡ和蛋白质合成的影响。结果化合物６Ｆ可强烈抑制ＳＰＣ－Ａ－１细胞的生长，２４ｈ的半数抑制浓度ＩＣ５０为２．３４μｍｏｌ·Ｌ－１；细胞周期分析显示６Ｆ作用细胞２４ｈ后，均能使细胞的Ｇ２／Ｍ期细胞比例升高，同时Ｇ０／Ｇ１期比例下降，并呈一定的剂量效应关系，６Ｆ对［３Ｈ］－ＴｄＲ、［３Ｈ］－ＵＴＰ和［３Ｈ］－Ｌｅｕ参入作用在培养２４ｈ后均有明显抑制作用，且呈剂量依赖关系。结论化合物６Ｆ明显抑制ＳＰＣ－Ａ－１细胞的生长，阻断细胞于Ｇ２／Ｍ期，抑制细胞ＤＮＡ、ＲＮＡ和蛋白质的合成。AIM To study the effects of compound 6F, one of the diterpenoids from Pteris Semipinnata L on cell cycle and the synthesis of DNA, RNA and protein of lung adenocarcinoma cell line SPC A 1. METHODS Cell viability was determined by using 3 (4,5 Dimethyl 2 thiazolyl) 2,5 diphenyl 2H tetrazolium bromide (MTT) test, the cell cycle was analysed by flow cytometry (FCM) and the synthesis of DNA, RNA and protein was observed by the TdR、 UTP and Leu incorporation. RESULTS Compound 6F strongly inhibited the growth of SPC A 1 cell, the IC 50 value was 2 34 μmol·L -1 after 24 h treatment. FCM analysis revealed that 6F could increase the percentage of cells in G 2/M phase and decrease those in G 0/G 1 phase after 24 h treatment, and in a dose dependent manner, while those in S phase changed a little. The assays of TdR、 UTP and Leu incorporation into DNA, RNA and protein respectively showed obvious inhibition dose dependently after exposure to 6F for 24 h. CONCLUSION Compound 6F remarkbly inhibited SPC A 1 cell line proliferation, arrested SPC A 1 cell in G 2/M phase and inhibited the synthesis of DNA, RNA and protein.

关 键 词：半边旗 二萜化合物 6F 肺腺癌细胞 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤] R979.1[医药卫生—临床医学]