葡萄胎Y染色体的出现率及其遗传学意义  

The presence of Y chromosome in hydatidiform mole and its genetic significance analysis

作　　者：张小为[1] 张丽珠[1] 周羡梅[1] 

机构地区：[1]北京医科大学第三医院妇产科

出　　处：《北京医科大学学报》1999年第2期149-151,共3页Journal of Peking University(Health Sciences)

摘　　要：探讨Ｙ染色体在葡萄胎中的出现率及其遗传学意义。方法；采用３对引物对大体病理完全性和部分性葡萄胎共４１例标本扩增Ｙ染色体长臂，短臂有和着丝粒区特异ＤＮＡ序列，结果：２８例完全性葡萄胎中Ｙ阳性者６例，１３例部分性葡萄胎中Ｙ染色体阳性者４例，统计学检验两类葡萄胎间Ｙ染色体出现率差异无显著性。To investigate the presence of Y chromosome in hydatidiform mole (HM) and its genetic significance. Methods: The specific DNA sequences of the long arm, short arm and the centromere area of Y chromosome in 41 HM samples were amplified by using PCR with three pairs of primers of DYZ Ⅰ, DYZ Ⅲ and SRY, respectively. Results: Y specific DNA sequences of DYZ Ⅰ, DYZ Ⅲ and SRY all positive were 6 (21. 4% ) in 28 complete hydatidiform moles (CHM) and 4 (30. 8 % ) in 13 partials' (PHM), respectively. Conclusion: Y presence rate is of no significance between CHM and in 13 PHM, which suggests that there is not much correlationship between pathologic and genetic classifications of HMs.

关 键 词：葡萄胎/遗传学 Y染色体 聚合酶链反应 基因扩增 

分 类 号：R737.33[医药卫生—肿瘤]