P21ras、PIK3CA在肝癌与肝硬化中的表达及意义  

Expression of P21ras and PIK3CA in human tissues of hepatocellular carcinoma and hepatic cirrhosis

作　　者：郑鹏飞[1] 李玉民[2] 李汛[3] 刘士源[3] 张全保[3] 何雯婷[3] 刘涛[2] 

机构地区：[1]兰州大学第一临床医学院,兰州730000 [2]兰州大学第二医院甘肃省消化系统瘤重点实验室,兰州730030 [3]兰州大学第一医院普外二科,兰州730000

出　　处：《国际外科学杂志》2010年第8期511-514,F0003,共5页International Journal of Surgery

基　　金：中国科学院（科发人教字[2009]236号） ＂西部之光＂人才培养计划资助项目

摘　　要：目的 研究P21ras和PIK3CA蛋白在乙肝病毒相关性肝细胞肝癌、乙型病毒性肝炎后肝硬化及正常肝组织标本中的表达差异、相关性及意义.方法 收集乙肝病毒相关性肝细胞肝癌组织标本34例、乙型病毒性肝炎后肝硬化37例、正常肝组织标本30例,用免疫组化检测P21ras和PIK3CA蛋白表达水平,并进行比较和相关性分析.结果 P21 ras在乙肝病毒相关性肝细胞肝癌、乙型病毒性肝炎后肝硬化及正常肝组织标本中的平均灰度值分别为138.86±2.9、145.34±2.06和152.07±1.17,与肝病的不同进展情况存在显著性差异（P＜0.01）.PIK3CA在乙肝病毒相关性肝细胞肝癌、乙型病毒性肝炎后肝硬化及正常肝组织标本中的平均灰度值分别为138.20±3.14、149.49±0.78和154.71±1.29,与肝病的不同进展情况存在显著性差异（P＜0.01）.在乙肝病毒相关性肝细胞肝癌组和乙型病毒性肝炎后肝硬化组中,P21ras和PIK3CA蛋白表达均有相关性,在乙肝病毒相关性肝细胞肝癌组中r=0.64,P＜0.05;乙型病毒性肝炎后肝硬化组中r=0.42,P＜0.05.结论 P21ras和PIK3CA蛋白在乙肝病毒相关性肝细胞肝癌、乙型病毒性肝炎后肝硬化组织的过表达提示二者参与了肝硬化、肝癌的发病过程.在乙肝病毒相关性肝细胞肝癌和乙型病毒性肝炎后肝硬化中可能存在着P21ras／PI3K的信号转导途径,从而促进肝细胞再生,发生肝硬化及癌变.Objective To investigate the expression of P21ras and PIK3CA proteins in hepatitis B virus-related hepatocellular carcinoma（HBV-HCC）, post-hepatitis B hepatic cirrhosis （HBV-hepatic cirrhosis）and normal hepatic tissues specimen, and their correlation between HBV-HCC and HBV-hepatic cirrhosis tissues.Methods Using immunohistochemistry, the expression of P21ras and PIK3CA proteins in 34 cases of HBV-HCC, 37 cases of HBV-hepatic cirrhosis and 30 cases of normal liver tissues specimen were detected and compared. Results The mean gray scales of P21ras protein in HBV-HCC, HBV-hepatic cirrhosis and normal hepatic tissue specimen were 138.86 ± 2.9, 145. 34 ± 2.06 and 152.07 ± 1.17 （P ＜ 0. 0l）, respectively, and were related to the progression of hepatopathy （P ＜0.01）. The mean gray scales of PIK3CA protein in HBV-HCC, HBV-hepatic cirrhosis and normal hepatic tissue specimen were 138.20 ± 3. 14, 149.49 ±0. 78 and 154.71 ± 1.29 （P ＜ 0.01）, respectively, and were related to the progression of hepatopathy （P ＜ 0. 01）.There were apparent correlation between P21ras and PIK3CA in HBV-HCC and HBV-hepatic cirrhosis respectively （r =0. 64, P ＜0. 05; r =0. 42, P ＜0. 05）. Conclusion The overexpression of P21ras and PIK3CA in HCC and hepatic cirrhosis tissue suggests that they participate in the tumorigenesis and development of hepatocellular carcinoma and hepatic cirrhosis, and there may be a signal transduction pathway of P21ras-PI3K in HBV-HCC and HBV-hepatic cirrhosis.

关 键 词：P21RAS蛋白 磷脂酰肌醇-3-激酶催化亚单位α 乙肝病毒相关性肝细胞肝癌 乙型病毒性肝炎后肝硬化 

分 类 号：R735.7[医药卫生—肿瘤]