丹参素异丙酯对离体大鼠缺血/再灌注损伤心肌的影响  

Effect of danshensu isopropyl ester on myocardial ischemia/reperfusion injury in rats

作　　者：程亮星[1] 岳云宵[1] 王世祥[2] 南叶飞[2] 郑晓晖[2] 戚敏[1] 于爽[1] 付润芳[1] 

机构地区：[1]郑州大学基础医学院药理学教研室,河南郑州450052 [2]西北大学生命科学学院,陕西西安710069

出　　处：《中国药理学通报》2010年第8期1045-1049,共5页Chinese Pharmacological Bulletin

基　　金：国家"重大新药创制"科技重大专项(No2009ZXJ09004-81);陕西省重大科技攻关项目(No2006kz10-G5)

摘　　要：目的观察丹参素异丙酯(IDHP)对离体大鼠模拟缺血/再灌注损伤心肌能量代谢及超微结构的影响。方法 Wistar大鼠80只,随机均分为8组:空白对照组、模型组、复方丹参滴丸(CDDP)50.00mg·L-1前保护及后保护组、IDHP4.00mg·L-1和0.04mg·L-1两个剂量的前保护及后保护组。利用Langendorff灌流技术,制备心肌缺血/再灌注损伤(MIRI)模型,各用药组分别在预灌及复灌时,给予相应浓度的药物。各组灌流结束后,7只心脏采用高效液相色谱仪测定其高能磷酸化合物(HEP)含量;其余3只心脏利用电镜观察心肌细胞超微结构。结果各用药组ADP、AMP及腺嘌呤核苷酸总量(TAN)升高(P<0.01,P<0.05);CDDP50.00mg·L-1前保护和后保护组、IDHP4.00mg·L-1前保护和后保护组的ATP含量升高(P<0.05)。电镜显示:IDHP4.00mg·L-1前保护、后保护组及CDDP50.00mg·L-1前保护、后保护组超微结构与空白对照组相似,损伤较轻;IDHP0.04mg·L-1前保护、后保护组心肌细胞超微结构的损伤有所改善,但损伤仍较严重。结论 IDHP无论前保护还是后保护均能改善缺血/再灌注损伤心肌能量代谢,减轻心肌细胞线粒体结构损伤。Aim To observe the effects of isopropyl-3-（ 3,4-dihydro-xyphenyl） -2-hydroxypropanoate（ IDHP） on the energy metabolism and ultrastructure of myocardium with ischemia/reperfusion injury. Methods 80healthy Wistar rats were randomly divided into 8 groups： control group,model group,compound danshen dropping prill （ CDDP） prevention group,CDDP protection group,two IDHP prevention groups with the dose of 4. 00 mg·L -1 and 0. 04 mg·L -1,two IDHP protection groups with the dose of 4. 00 mg·L -1 and 0. 04 mg·L -1,and each group had 10 rats. A myocardial ischemical/reperfusion injury （ MIRI） model was established with the Langendorff method. In each group,the high energy phosphates（ HEP） concentration was determined by HPLC in myocardium of 7 rats and the myocardial ultrastructure was observed by transmission electron microscope in myocardium of the other 3 rats. Results Compared with the model group,ADP, AMP and total adenine nucleotides（ TAN） levels were increased in all the medication groups（ P〈0. 01,P〈0. 05） ; ATP levels were increased in the two CDDP groups and the two IDHP groups with the dose of 4. 00 mg·L -1 （ P〈0. 05） . The damages of myocardial ultrastructure were light in two CDDP groups and two IDHP groups with the dose of 4. 00 mg·L -1. In two IDHP groups with the dose of 0. 04 mg·L -1,the damages of myocardial ultrastructure were lessened,though still severe. Conclusion IDHP can improve the energy metabolism of myocardium with ischemia/reperfusion injury,and lessen the damages of myocardial mitochondrion.

关 键 词：丹参素异丙酯 心肌缺血/再灌注损伤 高能磷酸化合物 透射电镜 能量代谢 心肌超微结构 

分 类 号：R-332[医药卫生] R322.11