鞘内注射p-MPPF对异氟烷镇痛作用的影响  

Influence of intrathecal injection of p-MPPF on the analgesic effects of isoflurane

作　　者：马涛[1] 袁力勇[2] 王丹[1] 戴体俊[1] 郭忠民[1] 张励才[1] 

机构地区：[1]徐州医学院麻醉学院,江苏徐州221004 [2]宁波市妇女儿童医院麻醉科,浙江宁波315012

出　　处：《中国药理学通报》2010年第8期1084-1087,共4页Chinese Pharmacological Bulletin

基　　金：宁波市自然科学基金资助项目(No2009A610140);江苏省高校自然科学基础研究资助项目(No09KJB320019)

摘　　要：目的探讨异氟烷(isoflurane,Iso)镇痛作用与小鼠脊髓5-HT1A受体的关系。方法昆明种小鼠腹腔注射Iso建立镇痛模型。分别以甩尾法、热板法、醋酸扭体法(15min内)评估小鼠鞘内注射5-HT1A受体拮抗剂p-MPPF(6μg和3μg)对Iso镇痛作用的影响。结果单独鞘内注射p-MPPF对小鼠甩尾潜伏期、热板痛阈、扭体次数无影响(P>0.05)。与Iso镇痛组(Iso组)相比,合用药组(Iso+M6组,Iso+M3组)甩尾潜伏期与热板痛阈均缩短(P<0.01或P<0.05);Iso+M6组扭体次数较Iso组增多(P<0.05),Iso+M3组无变化(P>0.05)。结论 Iso体表镇痛作用与激动小鼠脊髓5-HT1A受体密切相关。Aim To investigate the relationship be-tween the analgesic effects of isoflurane （ Iso） and the 5-HT1A receptor in the spinal cord of mice. Methods After the analgesic model of mice was established by intraperitoneal injection （ ip） of Iso,tail-flick test,hotplate test and acetic-acid-writhing test （ during 15 minutes） were used respectively to observe the influence of intrathecal administration 6 μg or 3 μg p-MPPF （ an antagonist of 5-HT1A receptor） on the analgesic effects of Iso. Results Intrathecal administration of p-MPPF alone had no effects on tail-flick latency （ TFL） ,pain threshold in hot-plate test （ HPPT） or writhing times （ WTs） （ P〈0. 05） . Compared with Iso analgesic group （ Iso group） ,the TFL or HPPT of co-administration groups （ Iso ＋ M6 group,Iso ＋ M3 group） shortened （ P〈0. 01 or P〈0. 05） ; the WTs of Iso ＋ M6 group increased （ P〈0. 05） whereas that of Iso ＋ M3 group was similar to Iso group （ P〈0. 05） . Conclusion These findings suggest that the surface analgesic effects of Iso are closely related to the excited 5-HT1A receptor in the spinal cord of mice.

关 键 词：异氟烷 镇痛 p-MPPF 5-HT1A受体 甩尾法 热板法 醋酸扭体法 脊髓 

分 类 号：R-332[医药卫生] R322.81