DBA/1小鼠胶原性关节炎模型建立方法及评价指标  

Establishing method and evaluation indexes of collagen-induced arthritis model in DBA/1 mice

作　　者：张玲玲[1] 刘云洁[1] 童彤[1] 吴颖其[1] 赵文娣[1] 李培培[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所.抗炎免疫药理学省部共建教育部重点实验室.抗炎免疫药物安徽省工程技术研究中心,安徽合肥230032

出　　处：《中国药理学通报》2010年第8期1108-1111,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30973543);安徽医科大学博士科研经费资助项目(NoXJ200808);安徽省研究实验基地优秀中青年科研带头人基金资助项目(No200622805)

摘　　要：目的建立DBA/1小鼠胶原性关节炎(collagen in-duced arthritis,CIA)模型及主要评价指标。方法鸡Ⅱ型胶原(typeⅡ collagen,CⅡ)免疫DBA/1小鼠诱导CIA,进行关节炎指数评分、足爪X线摄片、踝关节和脾脏病理检查及评分。结果免疫后d31,CIA小鼠足爪红肿,关节炎指数评分增高,d40～60为肿胀高峰;d35,体重下降;足爪X线片示关节变形,骨赘生成,骨质溶解;踝关节病理见滑膜组织增生,炎细胞浸润,血管翳出现;脾脏病理见生发中心增多,白髓淋巴样滤泡增生,边缘区细胞密度增大、红髓淤血。踝关节和脾脏病理评分明显高于正常小鼠。结论鸡CⅡ免疫DBA/1小鼠诱导CIA方法可靠,重复性好,CIA发病率高。关节炎指数、足爪X线片、踝关节和脾脏病理等是评价CIA模型的主要指标。Aim To establish method and main evaluation indexes of collagen induced arthritis （ CIA） model in DBA/1 mice. Methods CIA was induced by chicken type Ⅱcollagen （ C Ⅱ） in DBA/1mice. Arthritis was evaluated by arthritis index. X-ray of the paws was taken. Histology pathology of ankles and spleen was observed and scored. Results Immunization d31,the paws of CIA mice appeared red and swelling,the scores of arthritis index increased,the period of swelling peak was from d40 to d60; immunization d35,the weight of CIA mice began to decrease. X-ray of paws showed that the paw joints of CIA mice deformed,and there was osteophyte formation associated with osteolysis. Histo-logical pathology of ankle joints showed that the synovium of CIA mice were hyperplasia,cartilage was destroyed,pannus was formed,and inflammatory cells infiltrated into synovium. Histological pathology of spleen showed more germinal centers and lymphoid follicular hyperplasia were observed,cell density of lymphatic sheath increased,scores of ankle joints and spleen histological pathology in CIA mice were higher than those in normal mice significantly. Conclusions The methods of CIA model induced by chicken C Ⅱ in DBA /1 mouse were reliable and reproducible. CIA incidence was high. Arthritis index,X-ray of paws,ankle joints and spleen histological pathology and so on were the principal indexes of evaluation for CIA model.

关 键 词：胶原诱导性关节炎 小鼠 Ⅱ型胶原 模型 方法 评价指标 

分 类 号：R-332[医药卫生] R363-332