改性的配体交换型手性固定相的合成及应用  

Preparation and Application of Modified Ligand-exchange Chiral Stationary Phases

作　　者：宋瑞娟[1,2] 韩敏[1] 黄天宝[2] 

机构地区：[1]西安文理学院化学与化工系,陕西西安710065 [2]中国科学院成都有机化学研究所,四川成都610041

出　　处：《分析测试学报》2010年第8期867-870,共4页Journal of Instrumental Analysis

基　　金：西安文理学院院级重大课题资助项目(zd200904)

摘　　要：通过在巯丙基硅胶表面引入苯乙烯长链,再加入甲基丙烯酸缩水甘油酯在聚苯乙烯链端进行延长,用L-脯氨酸和L-羟脯氨酸进行修饰的方法合成了两种聚合物型配体交换型手性固定相(CSPⅣ和CSPⅤ),并采用6种氨基酸对映体考察了合成固定相的色谱拆分效果。结果表明,除DL-脯氨酸外,其余氨基酸对映体均具有相同的出峰顺序,即D-异构体优先出峰。与不含聚苯乙烯单元的配体交换型手性固定相相比,CSPⅣ和CSPⅤ具有更小的保留因子,更好的对映体选择性和更高的分离度。Two new polymeric chiral stationary phases（ CSPⅣ and CSPV ）for ligand-exchange chro- matography were synthesized based on the modification of L-proline and L-hydroproline with styrene and glycidyl methacrylate grafted on MPS silica gel. The enantioseparation capacity of CSP Ⅳ and CSP V for six DL-amino acids was investigated. The results indicated that CSPⅣand CSP V were successfully used for the enantioseparation of 6 DL-amino acids. Except for DL-proline, the other five DL-amino acids showed the same elution orders, and the retention of L-isomers was longer than that of D-isomers. Compared with the previous synthesized CSPs, the CSP Ⅳ and CSP V had less retention factors, better enantioselectivity and higher resolution.

关 键 词：手性固定相 配体交换色谱 对映体拆分 氨基酸 

分 类 号：O657.72[理学—分析化学] S963.731[理学—化学]