吴茱萸次碱对2型糖尿病肥胖大鼠的干预作用  

Intervention effects of rutaecarpine in type 2 diabetic obese rats

作　　者：聂绪强[1] 俞林花[1] 陈怀红[2] 赵同峰[2] 卞卡[3] 

机构地区：[1]上海中医药大学穆拉德中药现代化研究中心,上海201203 [2]浙江大学医学院附属二院,浙江杭州310009 [3]美国德克萨斯大学休斯敦医学院综合生物及药理学系,德克萨斯大学分子医学研究所,美国休斯敦TX77030

出　　处：《中国药理学通报》2010年第7期872-876,共5页Chinese Pharmacological Bulletin

基　　金：国家科技部“十一五”支撑计划项目(No2006BAIⅡB08-03);浙江省中医药科技计划项目(No2007CA052);上海市教委高校一氧化氮与炎症医学E研究院计划项目(NoE-04010);上海市科委国际技术转移项目(No08430711300),上海市科委中药现代化项目(No08DZ1972104)

摘　　要：目的观察吴茱萸次碱(rutaecarpine,Rut)对2型糖尿病肥胖大鼠(T2DM)的干预作用。方法用高脂饲料喂养结合一次性尾静脉注射小剂量链脲佐菌素(streptozotocin,STZ)30 mg.kg-1建立2型糖尿病肥胖大鼠模型。用Rut干预治疗,以二甲双胍片作为阳性对照。给药7 wk后观察对大鼠空腹血糖(FBG)、甘油三脂(TG),总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和血清炎症因子C-反应蛋白(CRP)等的影响,及肾脏、肝脏和胰脏的病理变化。结果 Rut能明显降低T2DM的TC、TG和LDL-C,升高HDL-C(P<0.05);Rut组CRP明显降低(P<0.05),病理切片观察Rut可以改善T2DM肥胖大鼠的肾、肝、胰的病理变化。但对血糖值没有明显降低(P>0.05),与二甲双胍组比较没有统计学意义(P>0.05)。结论 Rut能降低2型糖尿病肥胖大鼠的血脂,减轻炎症反应,改善肾、肝、胰的病理变化。Rut作为治疗T2DM代谢综合征的潜在药物值得进一步的研究。Aim To estimate the effect of rutaecarpine（Rut）on type 2 diabetic obese rats.Methods The type 2 diabetic obese rat model（T2DM）was generated with intravenous injection of streptozotocin（30 mg·kg^-1）and high-fat diet.The rats then were divided into 3 groups：rutaecarpine（Rut）-treated;metformin（Met;positive control）-treated;and control null-treated.7 weeks after treatment,the changes of fasting blood glucose（FBG）,fasting lipid profile including total cholesterol（TC）,triglyceride（TG）,low density lipoprotein-cholesterol（LDL-C）and high density lipoprotein-cholesterol（HDL-C）were observed.In addition,the pathological changes of kidney,liver and pancreas were examined.Results Rut treatment significantly decreased TC,TG,and LDL-C（P〈0.05）and increased HDL-C（P〈0.05）compared with T2DM non-treated group.The herb element also inhibited the levels of C-reactive protein（CRP;P〈0.05）.Rut improved pathological changes in kidney,liver,and pancreas of type 2 diabetic obese rats.But blood glucose levels was not statistically affected by Rut while compared with the Met group.Conclusions Rutaecarpine can benefit blood lipid profile,mitigate inflammation,and improve kidney,liver,pancreas pathologic status of type 2 diabetic obese rats.The further evaluation of rutaecarpine as a potential agent on diabetic metabolic syndrome is suggested.

关 键 词：吴茱萸次碱 2型糖尿病 SD大鼠 肥胖 胰岛素抵抗 高脂饲料 

分 类 号：R-332[医药卫生] R284.1