离体小动脉血管张力的研究方法  

A method for recording tension changes in small artery in vitro

作　　者：丁滢泂[1] 刘方[1] 朱依纯[1] 

机构地区：[1]及旦大宇上海医学院生理与病理生理掌系,上海200032

出　　处：《中国药理学通报》2010年第7期974-976,共3页Chinese Pharmacological Bulletin

基　　金：国家“重大新药创制”科技重大专项(No2009ZX09303-006);上海市中药现代化专项(No08DZ1971204)

摘　　要：目的通过建立离体小动脉血管张力的研究方法,为小血管的机能学和药理学研究提供有效的工具。方法分离大鼠肠系膜动脉,利用Chart5软件计算标准化的血管周径,然后通过自行编制的Excel文件计算血管内径和实验所需的初始张力设定值。随后对小血管施加各种刺激,用小血管肌动描记仪记录血管张力变化。结果小血管内径标准化提供小血管的管径数据,并为小血管初始张力的设定提供准确的参考数值,使实验条件标准化。小血管肌动描记仪能够记录各种刺激条件下小动脉血管张力的变化。结论成功建立离体小动脉血管张力的研究方法。Aim To establish a method for recording tension changes in small artery in vitro.Methods The vessel segments of intestine were dissected from the mesenteric artery of rats and mounted onto Myograph.The internal circumferences of the segments were normalized with Chart5 software.Then the internal diameters of the segments and initial setting of tension were calculated with self-made Excel file.Following these preparation steps, the tension changes induced by several stimulations were recorded by Myograph.Results Normalization procedure provided the data of lumen diameters of small arteries,which was convenient for initial setting of tension and guaranteed the uniformity of the experimental conditions.Myograph could record the tension changes of small arteries induced by several classic stimulations.Conclusion Precise recording method for tension changes in small arteries in vitro is successfully established.

关 键 词：小动脉 张力 肌动描记仪 内径 标准化 大鼠 

分 类 号：R-332[医药卫生] R-3333