胃癌术中腹腔游离癌细胞检测方法的对比研究  

Comparative Study on Detection of Peritoneal Free Cancer Cells of Gastric Surgery

作　　者：刘振芳[1] 韩晓鹏[1] 刘宏斌[1] 朱万坤[1] 苏琳[1] 

机构地区：[1]兰州军区兰州总医院普外科,兰州730050

出　　处：《中国普外基础与临床杂志》2010年第8期803-807,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：全军"十一五"计划面上项目资助(项目编号:06MA082)~~

摘　　要：目的探讨检测腹腔游离癌细胞的方法及临床意义。方法应用细胞学检查(PLC)、流式细胞术(FCM)、实时荧光定量RT-PCR分别检测50例胃癌患者术中腹腔冲洗液(腹水)中游离癌细胞、CK20蛋白和CK20mRNA表达阳性率,比较三种方法对腹腔游离癌细胞检测的敏感度。结果 50例胃癌患者腹腔冲洗液(腹水)中,PLC检测腹腔游离癌细胞阳性率为20.0%(10/50),FCM检测CK20蛋白表达阳性率为36.0%(18/50),实时荧光定量RT-PCR检测CK20mRNA表达阳性率为58.0%(29/50);实时荧光定量RT-PCR检测腹腔冲洗液(腹水)中CK20mRNA表达阳性率高于FCM检测CK20蛋白表达阳性率及PLC检测腹腔游离癌细胞阳性率(P<0.05或P<0.001);FCM检测腹腔冲洗液(腹水)中CK20蛋白表达阳性率与PLC检测游离癌细胞阳性率相比较,差异无统计学意义(P>0.05)。腹腔冲洗液(腹水)中检测CK20mRNA表达阳性率与肿瘤浸润深度、分化程度、TNM分期和淋巴结转移有关(P<0.05)。结论实时荧光定量RT-PCR检测CK20mRNA表达是检测腹腔游离癌细胞的有效方法。Objective To investigate the detection of peritoneal free cancer cells and its clinical significance. Methods The peritoneal free cancer cells,the positive rates of CK20 protein and CK20 mRNA expressions of peritoneal lavage fluid were detected by peritoneal lavage cytology （PLC）,flow cytometry （FCM） and real-time fluorescent quantitative RT-PCR in 50 cases of gastric cancer patients,respectively. The sensitivity of three kinds of detection method to peritoneal free cancer cells was compared. Results The positive rates of peritoneal free cancer cells,CK20 protein and mRNA expression of peritoneal lavage fluid were 20.0% （10/50）,36.0% （18/50） and 58.0% （29/50）,respectively. The positive rate of CK20 mRNA expression detected by real-time fluorescencequantitative RT-PCR in peritoneal lavage fluid was significantly higher than those of the CK20 protein expression detected by FCM and peritoneal free cancer cells detected by PLC （P0.05 or P0.001）. The difference of positive rate of CK20 protein expression and peritoneal free cancer cells was not significant （P0.05）. The positive rate of CK20 mRNA expression of peritoneal lavage fluid was related to the tumor invasion depth,differentiation degree,TNM stage,and lymph node metastasis （P0.05）. Conclusion Real-time fluorescence quantitative RT-PCR is an effective method for the detection of peritoneal free cancer cells.

关 键 词：胃癌 腹腔冲洗液 细胞学检测 流式细胞术 实时荧光定量RT-PCR 

分 类 号：R735.2[医药卫生—肿瘤]