运用Quantity One软件分析日本血吸虫不同发育时期蛋白组分差异  

Analysis of protein fractions differences of Schistosoma japonicum worms at various developmental period by using Quantity One software

作　　者：方会龙[1,2] 曾庆仁[2] 王俊杰[1] 曾铁兵[2,3] 喻容[2] 杨胜辉[2] 刘碧源[2,3] 刘彦[2,3] 蔡力汀[2] 

机构地区：[1]湘南学院基础医学课实验中心部,郴州423000 [2]中南大学医学细胞与分子生物学实验中心 [3]南华大学医学院病原生物学研究所

出　　处：《中国血吸虫病防治杂志》2010年第4期320-323,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30570952);湖南省自然科学基金(07JJ3031);湖南省教育厅项目(08C819)

摘　　要：目的应用Quantity One软件进行图像分析,阐明日本血吸虫不同发育时期蛋白组分的差异。方法以日本血吸虫尾蚴人工感染昆明鼠,分别收集感染前和感染后第8、12、16、20、24天和第28天虫体,用SDS-PAGE平行分离各时点虫体蛋白,用图像分析软件Quantity One4.4比较分析其区带差异。结果图像分析软件Quantity One4.4分析7个不同时点的虫体蛋白区带显示:区带数在0、8、12、16、20、24d和28d虫体中分别为27、30、33、32、31、36条和29条,各时点虫体特异性区带在0、8、12～24d和28d虫体中分别为7、4、8、3条,虫体特异性区带多为低表达量的条带。结论运用图像分析软件能够快速获得日本血吸虫不同发育时期蛋白泳道条带的分析图形,自动计算出蛋白区带数、区带光密度值大小和分子量。Objective To clarify the differences of protein fractions at different developmental periods of schistosoma japonicum by using Quantity One software.Methods The worms of Schistosoma japonicum were collected from infected Kunming strain of mice at different time points （0,8,12,16,20,24 and 28 days post-infection）.The different developmental individual soluble proteins were separated by SDS-PAGE after preparation.The band sizes and their differences were analyzed with Quantity One 4.4.Results Electrophoreses analysis of worm proteins from 7 different time points showed that the numbers of protein bands in 0-,8-,12-,16-,20-,24-and 28-day old worms were 27,30,33,32,31,36 and 29,respectively.Furthermore,there were 7,4,8,3 characteristic bands of the polypide in 0-,8-,12-to 24-and 28-day old worms,and most of the characteristic bands were low expression ones.Conclusions It is possible to obtain information from each lane and band efficiently through image analysis and calculate the protein zone number,the optical density value of bands and molecular weight automatically.

关 键 词：Quantity One软件 日本血吸虫 SDS-聚丙烯酰胺凝胶电泳 图像分析 蛋白组分 

分 类 号：R383.24[医药卫生—医学寄生虫学]