NOD2和TLR9激动剂诱导胃癌细胞SGC-7901合成IL-8的研究  

Study of NOD2 and TLR9 on release of IL-8 in gastric epithelial cells

作　　者：魏柏[1] 陈景三[1] 张书勤[1] 石奕[1] 马薇[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院肿瘤内科,湖北武汉430077

出　　处：《中国现代医药杂志》2010年第8期15-19,共5页Modern Medicine Journal of China

摘　　要：目的探讨TLR9和NOD2在胃癌细胞SGC-7901中表达及诱导IL-8合成过程中的相互作用及意义。方法用不同浓度的NOD2激动剂(MDP)和TLR9激动剂(未甲基化的CpG ODN)以不同时间间隔单独或联合作用于体外培养的胃癌细胞SGC-7901,用ELISA法测定上清中的IL-8,用RT-PCR和Western Blot测定TLR9和NOD2的表达。数据采用SPSS13.0进行统计分析。结果相对于未刺激组,MDP和未甲基化CpG ODN能使SGC-7901NOD2和TLR9mRNA和蛋白的表达增强;细胞培养上清中IL-8的分泌增强,并呈现时间依赖性和浓度依赖性,差异具有统计学意义(P<0.05),8h浓度差异无统计学意义,二者共同作用还能协同诱导SGC-7901合成IL-8,也呈现时间和浓度依赖性,差异具有统计学意义(P<0.05)。结论 NOD2和TLR9在胃癌细胞SGC-7901中有所表达,二者在诱导SGC-7901分泌炎性因子的过程中有协同作用。Objective To investigate the expression of NOD2 and TLR9 in gastric epithelial cells and its effect on production of IL-8 in SGC-7901.Methods In this study SGC-7901 was cultured in vitro and stimulated with NOD2 agonist Muramyl dipeptide （MDP） and TLR9 agonist unmethylated CpG ODN alone or combined.The mRNA and protein expression of NOD2 and TLR9 were examined in gastric epithelial cells with or without treatment of MDP and unmethylated CpG ODN using RT-PCR and Western Blot.Additionally the concentration in the culture supernatants of IL-8 was measured by ELISA.Results MDP and unmethylated CpG ODN up-regulate the expression NOD2 and TLR9 mRNA and protein level in SGC-7901.They induce the production of interleukin-8（IL-8） increasing in a time（P0.05） and concentration （P0.05） dependent manner.Additionally the results demonstrated that TLR9 and NOD2 had synergistic effect on increasing the production of IL-8 （P0.05）.Conclusion The NOD2 agonist MDP and TLR9 agonist unmethylated CpG ODN have effect on the expression of NOD2 and TLR9 in SGC-7901 and increasing the production of IL-8.NOD2 and TLR9 synergistically enhanced these effects.

关 键 词：TLR9 NOD2 胃癌细胞 协同作用 

分 类 号：R735.2[医药卫生—肿瘤]