抗AFP抗体双标记法及其产物特性测定  

Double Lable Method of Anti AFP Antibody and Determination of its Production Character

作　　者：周德南[1] 甘友全[1] 李志革[1] 吴英德[1] 黄秉琰[1] 

机构地区：[1]广西肿瘤防治研究所

出　　处：《广西医学》1999年第2期187-189,共3页Guangxi Medical Journal

摘　　要：采用氯胺丁改良法和过碘酸钠氧化法，进行抗ＡＦＰ多抗或单抗直接双标记１３１Ⅰ和ＭＭＣ，过Ｓｅｐｈａｄｅｘ－Ｇ５０柱并以ｒ计数仪与紫外分光光度仪同步监测分离、收集标记物。经测定多抗和单抗双标记物，标记比分别为７０．３ＭＢｑ∶ｍｇ∶２．２μｇ和５９．２ＭＢｑ∶ｍｇ∶１．８μｇ；放化纯度９８％和９７％；１３１Ⅰ利用率８３％和８８％；抗体回收率６２％和７４％；标记后与标记前相比抗体活性均无明显降低。置４℃５ｄ和１０ｄ时，抗体活性不受影响；放化纯度分别降为９５％和９２％、９１％和８８％。经裸鼠人肝癌模型放免显像，７２ｈ即见肿瘤区有放射性浓集，至１２０ｈ肿瘤显像最清晰。结果表明，多抗和单抗的双标记物的生物、理化性质稳定。为免疫导向治疗肝癌提供了一种新型的“双弹头”导向药物。Anti AFP polyclonal antibody(PcAb) or monoclonal antibody(McAb) was labeled directly with 131 Ⅰ and Mitomycine C(MMC) by modified Chloramin T method and sodium periodate oxidation method respectively The double labels were gained when the mixture passed through Sephadex G 50 column,which were monitored,separated synchronously with Gamma counter and ultraviolet spectrophotrometry The label ratioes of the labeled PcAb and McAb were( 131 Ⅰ∶anti AFP PcAb or McAb∶MMC)70 3MBq∶mg∶2 2μg and 59 2MBq∶mg∶1 8μg respectively The radiochemical purity,the rates of 131 Ⅰ utilization and antibody recovery were 98%,83%,62% and 97%,88%,74% respectively There was no loss of immunoreactivity before and after label In vitro,the immunoreactivity was not affected when the label antibody was stored at4℃ on day 5 and day 10 The radioimmunoimage in nude mice bearing liver cancer model showed that radioactive accumulation was seen in the tumor area on the day 3,and the tumor image was most distinct on day 5 These results indicate that the biological and physicochemical properties of the labeled antibody is stable It has provided a new type“dual warhead”targetting drug for RIT in the treatment of patients with PLC

关 键 词：抗甲胎蛋白抗体 双标记法 免疫导向疗法 肝癌 

分 类 号：R735.705[医药卫生—肿瘤] R730.51[医药卫生—临床医学]