嗅觉记忆相关蛋白的蛋白质组学研究  

Proteomic Analysis of Olfactory Memory Related Proteins in Mice

作　　者：郑君芳[1] 华琳[2] 张秋霞[3] 李德龙[1] 贺俊崎[1] 

机构地区：[1]首都医科大学生物化学与分子生物学系,北京100069 [2]首都医科大学生物信息学系,北京100069 [3]首都医科大学中医系,北京100069

出　　处：《高等学校化学学报》2010年第8期1535-1540,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30772573;30973405;30900247);北京市科技新星计划B类(批准号:2008B58);北京市优秀人才培养项目(批准号:20071D0501800253);首都医科大学科研基金(批准号:2008ZR01);首都医科大学基础临床合作基金(批准号:2007JL42;2007JL50;10JL26)资助

摘　　要：应用双向凝胶电泳结合质谱鉴定和数据库检索,分析比较了C57BL/6J小鼠在嗅觉训练和记忆测试后嗅球蛋白表达的差异,探讨了与嗅觉记忆相关的蛋白质.C57BL/6J小鼠经嗅觉训练后,可对相应的气味保持记忆能力,其嗅球蛋白表达存在明显差异,5种蛋白与嗅觉记忆形成显著相关.上述蛋白功能涉及神经发育、信号转导及细胞骨架和核苷酸代谢,其中神经发育和信号转导相关蛋白表达上调,而细胞骨架和核苷酸代谢相关蛋白表达水平降低.这些与嗅觉记忆形成相关的蛋白深化了对嗅觉记忆机制的认识,为研究和治疗认知相关疾病提供了新靶标.To investigate olfactory memory related proteins,olfactory bulb proteins from C57BL/6J mice after olfactory discrimination task were extracted and subjected to 2D gel electrophoresis.Protein quantification was performed and the differentially expressed spots were identified using mass spectrometry and Swiss-Prot database searching with MASCOT software.Olfactory bulb proteins from C57BL/6J mice after olfactory discrimination task were significantly different.Expression levels of 2 spots upregulated significantly;expression levels of 3 spots downregulated significantly.Functions of these identified proteins were involved in neurogenesis,signal transduction,cytoskeleton and nucleotide acid metabolism.These proteins improved insights into the olfactory memory mechanism and provided new targets for the study and treatment of cognitive diseases.

关 键 词：嗅觉记忆 嗅球蛋白 蛋白质组学 质谱 

分 类 号：O629[理学—有机化学] Q51[理学—化学]