反义6A8cDNA转染肿瘤细胞生物学行为的研究  

The effects of 6A8 cDNA transfection on biological behavior of Tumor cells

作　　者：刘玉琴[1] 张立新[1] 顾蓓[1] 马凤蓉[1] 赵雪梅[1] 薛克勋[1] 高进[1] 朱立平[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院

出　　处：《中华医学杂志》1999年第4期292-294,共3页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的探讨反义６Ａ８对肿瘤细胞恶性行为的影响。方法选用来自人鼻咽癌细胞系ＣＮＥ２Ｚ的高转移克隆ＣＮＥ２Ｌ２细胞，分别用ｐＲＣ／ＣＭＶ反义６Ａ８ｃＮＤＡ表达载体及空质粒转染并检测了反义６Ａ８ｃＤＮＡ转染、空载体转染及未转染细胞自身及与基质的粘附性、运动性和水解酶活性、体外侵袭性及体内生长转移能力。结果反义６Ａ８ｃＤＮＡ转染ＣＮＥ２Ｌ２细胞与基质的粘附性下降，其运动性和水解能力及体外侵袭及体内生长及转移能力也均下降。结论反义６Ａ８ｃＤＮＡ转染可明显降低肿瘤的恶性行为。Objective To investigate the effects of antisense 6A8 cDNA on the malignant behavior of tumor.Methods Highly metastatic clonal variant CNE 2L2 from nasopharygeal carcinoma cell line(CNE 2Z)was transfected with antisense 6A8 cDNA or mock plasmid. Their adhesion, locomotion, proteolytic ability, in vitro invasion, in vivo growth and metastasis were comparatively studied with the wild CNE 2L2 cells. Results Antisense 6A8 cDNA transfected CNE 2L2 showed less adhesion to FN and LN, weakened locomotive ability and proteolytic ability, retarded in vivo growth and much less metastasis. Conclusion Transfection of antisense 6A8 cDNA obviously decreased the malignant behavior of tumor cells.

关 键 词：反义CDNA 转染 肿瘤细胞 生物学行为 恶性行为 

分 类 号：R73－36[医药卫生—肿瘤]