癌基因c-erbB-2特异性核酶对胃癌细胞恶性表型的逆转  

Reversion of the malignant phenotype of gastric cancer by c erbB 2 specific ribozyme

作　　者：毕锋[1] 张学庸[1] 惠宏襄[1] 王成济[1] 陈峥[1] 樊代明[1] 

机构地区：[1]第四军医大学附属西京医院全军消化疾病研究所

出　　处：《中华医学杂志》1999年第4期295-297,共3页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的探讨ｃｅｒｂＢ２特异性核酶对胃癌细胞恶性表型的影响。方法根据计算机设计的人癌基因ｃｅｒｂＢ２特异性核酶ＲＺ１，合成ＲＺ１基因并构建其真核表达载体ｐＤＯＲＲＺ１，在ＬｉｐｏｆｅｃｔＡＭＩＮＥＴＭ的介导下转染胃癌细胞ＳＧＣ７９０１。流式细胞仪分析转染的胃癌细胞ｃｅｒｂＢ２产物Ｐ１８５的表达。在裸鼠体内观察转染胃癌细胞的致瘤性。结果在ＬｉｐｏｆｅｃｔＡＭＩＮＥＴＭ的介导下ｐＤＯＲＲＺ１成功地转染了胃癌细胞ＳＧＣ７９０１，经Ｇ４１８筛选出单克隆并称为ＳＧＣ／ＲＺ１。其蛋白产物Ｐ１８５的表达率被抑制达６２．７％；生长速度被抑制达５５％；在裸鼠体内的成瘤时间明显延迟，所成肿瘤的体积亦较对照组明显为小，表明其致瘤性明显减弱。结论ｃｅｒｂＢ２特异性核酶ＲＺ１对胃癌细胞的恶性表型有明显逆转作用。为胃癌的基因治疗提供了一个新的选择。Objective To probe the effect of c erbB 2 specific ribozyme to the malignant phenotype of gastric cancer. Methods The eucaryotic expresion vector of c erbB 2 specific ribozyme RZ1 was designed by computer and named pDOR RZ1.The transfection of the gastric cancer cell line SGC 7901 was mediated by Lipofect AMINE TM .Flow cytometry was used to analyse the expression of the c erbB 2 product P185. In vivo study of tumorogenecity of the transfected cells was performed in nude mice. Results pDOR RZ1 was successfully transfected into the gastric cancer cell line SGC 7901 and then the single clones were selected by G418 and named SGC/RZ1. Flow cytometry analysis showed that the expression of P185 was suppressed by 62.7%. The growth rate of SGC/RZ1 was inhibited by 55%. The tumor forming time in SGC/RZ1 in nude mice was delayed remarkably and the tumor size was also much smaller than that in the control group, indicating the inhibition of the tumorogenecity of SGC/RZ1 in nude mice. Conclusion c erbB 2 specific ribozyme is very efficient in reversing the malignant phenotype of gastric cancer. This might provide a new approach for gene therapy of gastric cancer.

关 键 词：胃肿瘤 癌基因 C-ERBB-2 基因治疗 

分 类 号：R735.205[医药卫生—肿瘤]