长期骨髓培养、白细胞介素2、干扰素α_(2a)体外净化慢性粒细胞白血病骨髓的研究  

Study on Purging Chronic Myeloid Leukemia Marrow with the Combination of Long Term Bone Marrow Culture, Interleuk 2 and Interferon α 2a

作　　者：陈伦华[1] 李秀珍[1] 陈济民[1] 敖绪斌[1] 仲照东[1] 高清平[1] 谢燕[1] 陈友华[1] 夏虹[1] 

机构地区：[1]湖北医科大学附属第一医院血液科

出　　处：《同济医科大学学报》1999年第2期123-125,共3页Acta Universitatis Medicinae Tongji

基　　金：湖北省卫生厅资助

摘　　要：用长期骨髓培养（ＬＴＢＭＣ）和白细胞介素２（ＩＬ－２，１０００Ｕ／ｍｌ），干扰素α２ａ（ＩＦＮ－α２ａ，１０００Ｕ／ｍｌ）不同的组合方案，对１０例慢性粒细胞白血病（ＣＭＬ）骨髓进行体外净化培养２周。结果发现：①单用ＬＴＢＭＣ体系其净化效果差；②加入ＩＬ－２后，对ＣＭＬ的ＣＦＵ－ＧＭ以及ｐｈ＇（＋）细胞有明显的杀伤作用（Ｐ＜０．０１，Ｐ＜０．０５）对正常的ＣＦＵ－ＧＭ则无影响；③ＩＦＮ－α２ａ在ＬＴＢＭＣ体系中抑制ｐｈ＇（＋）细胞生长而不影响ＣＭＬ和正常的ＣＦＵ－ＧＭ；④ＬＴＢＭＣ、ＩＬ－２、ＩＦＮ－α２ａ三者净化ＣＭＬ骨髓具有协同作用。结果提示：ＬＴＢＭＣ、ＩＬ－２、ＩＦＮ－α２ａ联合净化效果好。Bone marrow cells from 10 patients with chronic myeloid leukemia (CML) were purged with different combination of long term bone marrow culture (LTBMC), interleukin 2(IL 2,1 000 U/ml) and interferon α 2a (IFN α 2a ,1 000 U/ml) for two weeks in vitro . It was found that: ①The purging effect of single LTBMC was poor; ②Significant killing effects on CFU GM of CML and ph'(+) cells were found after addition of IL 2 in LTBMC ( P <0.01, P <0.05). IL 2 could not affect the CFU GM of normal control; ③IFN α 2a could inhibit the growth of ph'(+) cells without affecting CFU GM of CML and normal in LTBMC system. ④ LTBMC, IL 2 and IFN α 2a might exert a synergetically purging effect on ph'(+) CML cells. These results suggest that purging bone marrow with combination of LTBMC, IL 2 and IFN α 2a might have good purging effect, and could be used for purging CML marrow in clinical practice.

关 键 词：骨髓培养 白细胞介素2 干扰素Α 白血病 CML 

分 类 号：R733.72[医药卫生—肿瘤]