膀胱癌中Twist蛋白表达与上皮-间质转化的关系  

Relationship between epithelial-mesenchymal transition and Twist expression in bladder cancer

作　　者：张学能 李叶红 邢诗安[2] 朱朝辉[3] 王智宇[3] 吕磊[3] 何远桥[3] 刘朝[2] 祝建芳[2] 

机构地区：[1]湖北省阳新县人民医院泌尿外科,435200 [2]华中科技大学同济医学院附属协和医院中心实验室 [3]华中科技大学同济医学院附属协和医院中心泌尿外科

出　　处：《中华实验外科杂志》2010年第8期1134-1136,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30872561）

摘　　要：目的 探讨膀胱癌中上皮-间质转化因子Twist表达及其与上皮-间质转化及病理参数之间的关系.方法 应用E-钙黏蛋白和N-钙黏蛋白的表达来检测上皮-间质转化的发生.采用免疫组织化学SABC法、实时定量聚合酶链反应（PCR）和Western blot法观察45例膀胱癌组织和5例正常膀胱组织中Twist蛋白、E-钙黏蛋白和N-钙黏蛋白的表达.结果 45例膀胱癌组织和5例正常膀胱黏膜中Twist阳性率分别为26.66%和0,随着膀胱癌的病理分级及临床分期的增高,Twist的表达率逐渐升高.且Twist在有淋巴转移中的表达明显高于无淋巴结转移的膀胱癌.Twist的表达与E-钙黏蛋白和N-钙黏蛋白的表达显著相关.结论 Twist蛋白的表达对上皮-间质转化有重要影响,可为膀胱癌侵袭转移的研究和患者的预后判断提供参考.Objective To investigate the relaitonship between the immunohistoehemical expression of Twist and epithelial-mesenchymal transition in bladder cancer. Methods The epithelial-mesenchymal transition was assessed by the expression of E-cadherin and N-cadherin. The expression of E-cadherin and N-cadherin in formalin-fixed and paraffin-embedded tissues and fresh tissue blocks from 49 pa- tients with bladder cancer was detected by immunohistochemistry, real-time polymerase chain reaction （PCR） and Western blotting. Results The expression of Twist protein was detected in 14 cases （26. 66% ） of bladder cancer. Twist positive rate was associated with the pathological parameters. The expression of Twist was closely associated with epithelial-mesenchymal transition and infiltration and metastasis. Conclusion Twist has an effect on epithelial-mesenehymal transition in bladder cancer. Twist may play an important role in the infiltration and metastasis of bladder cancer.

关 键 词：膀胱癌 TWIST蛋白 转移 

分 类 号：R737.14[医药卫生—肿瘤]