水样中痕量铅的荧光动力学分析法  

Fluorescence Kinetics Analysis Method for Trace Lead in Water

作　　者：邢荣秀[1] 于倩[2] 刘嘉坤[1] 张树焕[1] 张媛媛[1] 

机构地区：[1]沧州医学高等专科学校药学系分析化学教研室,河北沧州061001 [2]沧州市中心医院功能检验科心电图室,河北沧州061001

出　　处：《环境与健康杂志》2010年第8期709-711,共3页Journal of Environment and Health

摘　　要：目的建立水样中痕量Pb2+的荧光动力学分析法。方法痕量Pb2+对过氧化氢氧化二氯荧光素(DCF)的反应具有较强的催化作用,使体系的荧光强度减弱得更为明显。反应体系的激发波长和发射波长分别为491和517nm,通过测定痕量Pb2+对过氧化氢氧化DCF后DCF的荧光强度来检测Pb2+的含量。结果 Pb2+的线性范围为4.0～100.0μg/L,检出限为3.1μg/L,其线性回归方程为:ΔF=828C+1.26,r=0.9990,平均回收率为91.3%～98.1%,RSD为4.0%。结论该法具有简便、快速、灵敏等优点,适合水样中痕量铅的测定。Objective To establish a new fluorescence kinetics analysis method for the trace lead in water sample. Methods Trace Pb^2＋may present a strong catalytic effect on the reaction of hydrogen peroxide oxidation of dichlorofluorescein （DCF）,when adding trace Pb^2＋, the fluorescence intensity weakened more obviously, through the determination of fluorescence intensity to determine the Pb^2＋ content. In the reaction system, the excitation wavelength and emission wavelengths were 491 nm and 517 nm respectively. Results The linear range of Pb^2＋ was 4.0-100.0 μg/L, the limit of detection was 3.1 μg/L, the linear regression equation was： △F=828 C （μg/ml）＋1.26, the correlation coefficient was 0.9990. Conclusion This method is simple, fast, sensitive and annlicable to the determination of trace lead in water.

关 键 词：荧光动力学分析法 水 铅 二氯荧光素 

分 类 号：O657.7[理学—分析化学]