2-乙酰氨基芴诱发大鼠肝癌变过程中rasp21、AFP的原位表达  

In Situ Expession of Rasp21 and AFP during Hepatocarcinogenesis in Rats Induced by 2-Fluorenylacetamide

作　　者：韩新华[1] 施公胜[1] 王淼[1] 顾美珍[1] 

机构地区：[1]南通医学院附属医院

出　　处：《河南肿瘤学杂志》1999年第3期182-184,共3页Henan Journal of Oncology

摘　　要：目的探讨了rasp21、AFP在2-乙酸氨基芴（2－FAA）诱发的大鼠肝癌变过程中的分布情况及关系。方法rasp21和AFP采用免疫组化S－P法被检测及分析。结果诱癌早期增生的肝细胞及变异肝细胞灶中即显示rasp21和AFP的过度表达，并随增生肝细胞结节的形成和演变二者过度表达细胞增多且相伴而存，由此提示，两者与大鼠肝癌发生有密切关系，rasp21过度表达直接参与了大鼠肝癌的启动和演进。结论同时表达AFP,rasp21的细胞和病变是较具特异的癌前病变，p21与AFP的联合表达可为人类肝癌提供一个参考诊断标志。Objective To study the expession and relationship of rasp21 and AFP during the hepatocarcinogenesis in rats induced by 2- FAA (2-Fluorenylacetamide). Methods Rasp21 and AFP were detected by using immunohistochemical S -Pmethod .Results Overexpression of rasp21 and AFP was presented in the proliferative hepatocytes and al temated hepatocytes foci during foci during the formation and progression of hyperplastic hepatocytic nodules,the overexpression cells ofboth were increased and often accompanied each other.Results Both rasp21 and AFP had close ralationship with hepato carcinogenesis and the overexpression of rasp21 was directly involved in the initiation and progression of hepatocarcinoma.the combined expression of rasp21 and AFP could be used as a consulative marker for the early diagnosis of humam hepato carcinoma.[

关 键 词：肝肿瘤 癌基因蛋白 致癌物 大鼠 

分 类 号：R735.702[医药卫生—肿瘤]