SYBR Green实时定量PCR检测急性髓系白血病患者BAALC基因表达  

Detection of BAALC in patients with acute myeloid leukemia by real-time reverse transcriptase-polymerase chain reaction

作　　者：唐洁[1] 申林[2] 李柏青[1] 赵皓[3] 余晾[3] 郑洪波[3] 

机构地区：[1]蚌埠医学院免疫学教研室感染与免疫安徽省重点实验室,安徽蚌埠233004 [2]蚌埠医学院中心实验室,安徽蚌埠233030 [3]蚌埠医学院第一附属医院中心实验室,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2010年第7期656-659,共4页Journal of Bengbu Medical College

基　　金：安徽省高校省级重点实验室重点科研项目(2004sys008);蚌埠医学院科研基金资助项目(编号:BY0705)

摘　　要：目的：观察SYBR Green相对定量逆转录聚合酶链反应（RQ-PCR）方法检测急性髓系白血病（AML）患者外周血单个核细胞（PBMC）中脑和急性白血病细胞胞质（BAALC）基因mRNA的表达水平。方法：分离12例初发AML患者和5名健康成年人PBMC,以白血病细胞株K562为阳性对照,提取细胞总RNA并逆转录为cDNA,SYBR Green相对定量RQ-PCR检测BAALC基因表达水平。结果：健康成年人PBMC中检测不到BAALC mRNA的表达,12例AML患者中,有8例BAALC mRNA高表达,与K562比较,其相对值为K562的0.8~5.6倍。结论：用SYBR Green相对定量RQ-PCR可以检测到AML患者PBMC中BAALC基因的表达,该方法稳定、敏感、可靠。Objective：To detect the mRNA expression of brain and acute leukemia,cytoplasmic（BAALC） in perpheral blood mononuclear cells（PBMC）,BAALC of patients with acute myeloid leukemia（AML） by comparative real-time reverse transcriptase-polymerase chain reaction（RQ-PCR） with SYBR Green.Methods：PBMC was obtained from 12 patients with AML and 5 healthy donors,from which total RNA was extracted and reverse transcribed into cDNA.The leukemia cell lines K562 cells was selected as positive control.The expression of BAALC was detected by RQ-PCR with SYBR Green assay.Results：The BAALC mRNA could not be detected in PBMC of healthy donors.However,in 12 patients with AML,it was highly expressed in 8 of them.The relative values were 0.8-5.6 compared with K562 cells.Conclusions：The expression of BAALC in PBMC of patients with AML could be detected by SYBR Green comparative RQ-PCR.It was a steady,sensitive and reliable assay method.

关 键 词：基因表达 白血病 细胞胞质基因 逆转录聚合酶链反应 

分 类 号：Q343.1[生物学—遗传学] R733.71[医药卫生—肿瘤]