p53基因突变与EB病毒相关性鼻咽部T细胞淋巴瘤  

p53 Gene Mutation and Epstein-Barr Virus Associated Nasopharyngeal T-cell Lymphomas

作　　者：高子芬[1,2,3] 姜芬[1,2,3] 潘增刚 王洪海 田君才[1,2,3] 

机构地区：[1]北京医科大学病理学系 [2]解放军空军总医院病理科 [3]甘肃省酒泉地区医院

出　　处：《中国肿瘤临床》1999年第6期429-432,共4页Chinese Journal of Clinical Oncology

基　　金：卫生部科学研究基金

摘　　要：目的：了解ｐ５３基因突变与埃伯斯坦－巴尔病毒（ＥＢＶ）相关性鼻咽部Ｔ细胞淋巴瘤（ＮＰＴ）的关系。方法：应用免疫组织化学方法（ＳＡＢＣ），原位杂交（ＩＳＨ），ＰＣＲ－ＳＳＣＰ对２６例确诊为鼻咽部Ｔ细胞淋巴瘤的病例进行ｐ５３蛋白及基因的检测。结果：ＥＢＶ编码的小ＲＮＡ探针（ＥＢＥＲ）１７／２６例阳性（６５４％）；ｐ５３蛋白１３／２６例阳性（５０％）；对其中１２例进行ｐ５３外显子５，７，８的ＰＣＲ－ＳＳＣＰ检测，发现ｐ５３突变７／１２例（５８３％）阳性，ＥＢＶ阳性组５／８例阳性（６２５％），阴性组２／４例（５０％）阳性，外显子５突变４／１２例（３３３％）阳性，外显子７突变１／１２例（８３％）阳性，外显子８突变３／１２例（２５％）阳性，其中１例同时发生５，８两个外显子突变；ＥＢＥＲ／ｐ５３蛋白表达同时发生有１１例，不发生７例，ＥＢＥＲ＋／ｐ５３－６例，ＥＢＥＲ－／ｐ５３＋２例；ＥＢＥＲ表达（ＩＳＨ）与ｐ５３基因突变（ＰＣＲ－ＳＳＣＰ）同时发生５例，不发生２例，ＥＢＥＲ＋／ｐ５３－（ＰＣＲ－ＳＳＣＰ）３例，ＥＢＥＲ－／ｐ５３＋（ＰＣＲ－ＳＳＣＰ）２例；以ｐ５３蛋白检测为标准，ｐ５３基因突变（ＰＣＲ－ＳＳＣＰ?Objective: To study the correlation of p53 gene mutation and Epstein-Barr virus (EBV) associated nasopharyngeal T-cell lymphoma (NPT) Methods: p53 protein and gene were determined in 26 cases of EBV associated nasopharyngeal T-cell lymphoma by immunohistochemistry (SABC), in situ hybridization (ISH), PCR-SSCP Results: EBV encoded mRNA (EBER) was found in tumor cells in 17/26 cases,so were p53 protein in 13/26 cases and p53 gene mutation in 7/12 cases (exon 5: 4/12, exon 7:1/12 , exon 8: 3/12) Co-expression of EBER and p53 protein was found in 6 cases, whereas in 3 cases either one was identified and in 3 cases there was no expression of p53/EBER Simultaneous occurrence of EBER and p53 gene mutation was observed in 5 cases, whereas in 2 cases neither was found The concidence rate between p53 gene mutation and p53 protein expression was 83% Conclusion: The results showed that no clear relationship was found between p53 gene mutation and EBV associated nasopharyngeal T-cell lymphoma

关 键 词：EBV P53 EBER 鼻咽部 T细胞淋巴瘤 

分 类 号：R739.63[医药卫生—肿瘤]