纳米均相时间分辨荧光免疫法检测脱氧雪腐镰刀菌烯醇方法的建立  

Quantitative determination of deoxynivalenol using light initiated chemiluminescence assay

作　　者：周彬[1] 高雷 金坚 陈蕴 黄飚[1] 赵卫国 

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室,江苏无锡214063 [2]江南大学医药学院,江苏无锡214063 [3]博阳生物科技(上海)有限公司,上海200000

出　　处：《食品工业科技》2010年第8期338-342,共5页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863)(2008AA10Z415);江南大学食品科学与技术国家重点实验室目标导向课题(SKLF-MB-200801)

摘　　要：目的:利用抗脱氧雪腐镰刀菌烯醇(DON)多克隆抗体,采用纳米均相时间分辨荧光免疫法测定技术(AlphaLISA)建立间接竞争DON定量检测方法。方法:DON-BSA包被发光微粒,生物素化羊抗兔抗体与包被有链霉素的感光微粒共同构成检测试剂,优化检测条件并评价检测性能。结果:该方法的灵敏度为0.007ng/mL,检测范围为0.007～100ng/mL,批内批间变异系数均<5%。不同样品添加回收实验:玉米、小麦、啤酒回收率分别为84%～110%、67%～100%、74%～100%。结论:DON-AlphaLISA是目前DON检测中最灵敏的方法之一,该分析方法稳定性好,可测范围宽,具有很好的应用前景。Objective： An indirect competitive light initiated homogeneous time-resolved fluoroimmunoassay （ AlphaLISA） was established by using anti-DON polyclonal antibody and coating antigen DON-BSA for detection of deoxyhivalenol in cereal.Method： DON-BSA was coated on chenilum inescent particles,the AlphaLISA kit also contained sensitizer particles coated with strep tavidin.The optinal test conditions and analytical performance of the method were studied.Results： Working concentration range from 0.007 to 100ng /mL and the sensitivity for detection was 0.007ng /mL.The intra-and inter-assay CV of the assay were both below 5%.The recoveries of determination for deoxynivalenol spiked in corn,wheat,beer were 84%～ 110%、67%～ 100%、74%～ 100% . Conclusions： This AlphaLISA method is a good method with high sensitivity for quantitative analyzing DON in cereal.

关 键 词：脱氧雪腐镰刀烯醇 生物毒素 纳米均相时间分辨荧光免疫法(AlphaLISA) 

分 类 号：Q939.91[生物学—微生物学]