大鼠慢性主动脉血管移植排斥反应模型的建立  

The establishment of chronic rejection model of rat＇s aortic transplantation

作　　者：唐晶晶[1] 林艳娟[1] 

机构地区：[1]福建医科大学附属协和医院血液科,福州350000

出　　处：《中华显微外科杂志》2010年第4期301-304,357,共5页Chinese Journal of Microsurgery

基　　金：基金项目：福建省自然科学基金资助项目（2007J0084）

摘　　要：目的建立一种大鼠慢性主动脉血管移植排斥反应模型。方法以雄性Lewis鼠为供者．雌性Brown Norway鼠（BN鼠）为受者建立移植模型，截取Lewis鼠胸主动脉1．5cm，移植于BN鼠腹主动脉段行端端吻合。术后1周每天量体重，观察有无弓背、掉毛、腹泻、精神萎靡等症状，术后第2周起改为每周观察一次．术后8周取移植血管行病理切片、苏木精－伊红（HE）染色及免疫组化检查，观察血管内膜增厚情况及管壁上CD3、CD68、α－actin表达。结果所有手术均于2h内完成，围手术期死亡数为零。所有的受鼠均存活超过8周。术后受鼠出现弓背、掉毛、体重减轻等症状，第4、5天体重开始回升，1周后基本恢复至术前水平。术后56d取移植血管，病理切片可见血管内膜较未移植血管明显增厚，免疫组化可见管壁出现大量棕黄色CD3、CD68、α－actin表达阳性细胞。结论移植后的血管血管内膜明显增厚．管壁上出现大量CD3、CD68、α－actin表达阳性细胞均为慢性排斥反应表现，表明该法建立的大鼠主动脉血管移植模型可为研究慢性排斥反应的发生、进展和干预治疗提供良好的平台。Objective To establish an aortic transplantation model in the rat. Methods A thoracic aorta to abdominal aorta interposition transplant model in the rat was established. The male Lewis rats were used as donors and the female Brown Norway （BN） rats were used as recipients.Intercepted the thoracic aorta of lewis rats about one and a half centimeter. Transplanted it on the abdominal aorta of BN rats. The activity and weight change of BN rats were observed everyday until 1 week after transplantation. Then observed these index one time a week. The transplanted aortas were harvested at 8 weeks after transplantation. The intimal thickening were observed by pathological. The expression of CD3 ,CD68 and α-actin on transplanted aorta were observed by immunohistochemistry. Resuts All operations were finished within 2 hours. No recipients died during operation. All recipients could survival more than 8 weeks. The symptoms of bowing back, hair shedding and weight loss was appeared after transplantation. The weight of the rats were upturn at 4 or 5 days after transplantation. About 1 week after transplantation, all situations of the rats were recovery to the lever before transplantation. 8 weeks after transplantation the intimal thickening of the transplanted aortas were observed obviously by pathological. And the expressions of CD3, CD68 and α-actin on aortic allograft were observed. Conclusion The intimal thickening and the expressions of CD3, CD68 and α-actin on aortic allograft were typical expressions of chronic rejection.So this aortic transplantation model is feasible and reliable for studying chronic rejection.

关 键 词：主动脉 血管移植 排斥反应 动物模型 

分 类 号：R-332[医药卫生]