XAGE-1基因在肺癌组织中的表达及其与临床特点的关系  

Association of XAGE-1 Gene Expression with Clinical Characteristics of Lung Cancer

作　　者：赖群 张立堂 孙彦 

机构地区：[1]山东省枣庄矿业集团中心医院心胸外科,枣庄277100

出　　处：《中国肺癌杂志》2010年第8期786-789,共4页Chinese Journal of Lung Cancer

摘　　要：背景与目的研究证实XAGE-1作为一种癌胚抗原在多种肿瘤中高表达。本研究旨在探讨XAGE-1基因在肺癌组织中的表达水平及其与临床特点的关系。方法肺癌患者85例,提取肺癌组织和癌旁组织总RNA,巢式PCR反应扩增XAGE-1四种剪接体基因,分析基因表达情况及其与临床特点的关系。结果 32.94%(28/85)肺癌患者癌组织阳性表达XAGE-1基因,在各种类型肺癌中,59.46%(22/37)的腺癌与21.74%(5/23)鳞癌患者癌组织阳性表达XAGE-1基因;XAGE-1b基因的表达与肺癌病理类型有相关性(P<0.05),腺癌阳性表达率明显高于鳞癌,而与肺癌的性别、年龄及临床分期无相关性(P>0.05)。结论 XAGE-1基因在肺癌尤其腺癌组织中高表达,该基因可以作为免疫治疗的靶基因。Background and objective XAGE-1 is a cancer-testis （CT） antigen which was demonstrated to be expressed at a significant frequency and to be immunogenic in some tumors.The aim of this study is to explore the association between XAGE-1 gene expression and the clinical characteristics of lung cancer.Methods Tumor tissue and adjacent lung tissue samples from 85 patients were screened for expression of the four XAGE-1 transcript variants by nest PCR.The correlations between XAGE-1b gene expression and several clinical characteristics were analyzed.Results 32.94% （28/85） of lung cancer samples were positive for XAGE-1 gene.59.46% （22/37） of the adencarcinoma samples and 21.74% （5/23） of the squamous cell carcinoma samples were positive for one of the four XAGE-1 transcript variants.The frequent of XAGE-1b gene in adencarcinoma was much higher than that in squamous cell carcinoma.There were not any important correlation between XAGE-1b gene expression and clinical characteristics,such as gender,age and clinical stage.Conclusion XAGE-1 gene is highly expressed in lung adenocarcinoma and XAGE-1 may be a promising immunotherapeutic target for lung cancer.

关 键 词：XAGE-1基因 CT抗原 肺肿瘤 临床特点 

分 类 号：R734.2[医药卫生—肿瘤]