人原发性肝细胞癌和癌旁组织HBV X基因产物表达和ets-2、IGF-Ⅱ、C-myc及N-ras表达的对比研究  

THE EXPRESSION OF HBV X GENE AND ets-2, IGF-Ⅱ, C-myc AND N-ras IN HEPATOCELLULAR CARCINOMA AND TUMOR-ADJACENT TISSUES

作　　者：连兆瑞[1] 吴孟超[1] 万大方[2] 徐国威[2] 周筱梅[2] 顾健人[2] 

机构地区：[1]第二军医大学附属长海医院肝胆外科 [2]上海市肿瘤研究所分子生物学和癌基因研究室

出　　处：《上海医学》1990年第3期153-158,共6页Shanghai Medical Journal

摘　　要：本文采用RNA杂交和免疫印迹法检测了7对原发性肝癌(PHC)和癌旁组织及血清中HBxAg、ets-2、IGF-Ⅱ、C-myc和N-ras的表达。结果表明,在肝组织内HBxAg表达为17和28Kd的特异性条带。在3例血清中,可见到17Kd HBxAg。在有HBxAg表达的肝组织内,常有多种痛基因如ets-2,IGF-Ⅱ、C-myc和/或N-ras同时表达增加的现象,提示HBxAg的非特异性反式激活作用。在部分癌旁组织有癌基因表达高于癌组织的现象。文内讨论了HBxAg与癌基因表达相互关系及在PHC发生中的可能作用。The expression of HBV X gene and ets-2, IGF-Ⅱ, C-myc and N-ras was studied in 7 pairs of primary hepatocellular carcinoma (PHC) and tumor-adjacent tissues, using RNA hybridization and immune blot methods. The results showed that specific 17 and 28 Kd HBV X gene product-HBxAg existed in a part of PHC and tumor-adjacent tissues. The 17 Kd HBxAg was also detected in the sera of 3 patients who had 17 Kd HBxAg in the liver tissues. Multiple expression of oncogenes such as ets-2, C-myc and N-ras was also detected in PHC and tumor-adjacent tissues that had HBxAg expression, indicating that HBxAg might function as a transactivator in the course of intracellular proto- oncogene activation. It is also observed that in some tumor-adjacent tissues the expression of oncogenes such as ets-2, C-myc and N-ras was stronger than that in corresponding PHC. The relation of HBxAg to the expression of ets-2, IGF-Ⅱ, C-myc and N-ras and their respective roles in the carcinogenesis of PHC are discussed.

关 键 词：肝癌 HBXAG 基因表达 肝肿瘤 

分 类 号：R735.702[医药卫生—肿瘤]