眼镜蛇毒细胞毒素的分离、纯化及其抗癌活性  

Isolation, Purification and Anticancer Activity of the Cytotoxin from Cobra Venom

作　　者：王锡锋[1] 曹宜生[1] 刘一平 宾曼蓉 

机构地区：[1]广州军区联勤部军事医学研究所,510507

出　　处：《中国生化药物杂志》1999年第3期131-133,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的：研究眼镜蛇毒细胞毒素的抗癌活性。方法：应用Ｓｅｐｈａｄｅｘ　 Ｇ１００和 ＣＭ－Ｓｅｐｈａｒｏｓｅ ＦＦ柱层析，从眼镜蛇毒中分离、纯化细胞毒素组分，用ＭＴＴ法测定该组分对体外培养的人癌细胞的细胞毒性作用。结果：眼镜蛇毒细胞毒素对人癌细胞ＳＧＣ－７９０１、Ｂｅｌ－７４０２、Ｋ５６２和Ｕ９３７的抑制作用呈良好的量效关系，半数抑制浓度分别为 ４．１０、 ２． ０８、 ０． ２９和 ０．１７ μｇ／ｍｌ。结论：眼镜蛇毒细胞毒素对体外培养的人癌细胞有很强的杀伤作用。Purpose: The aim was to study the anticancer activity of cobra (Naja naja atra) venom cytotoxin. Methods: The cytotoxin was separated and purified from cobra venom through Sephadex G100 and CM-Sepharose FF column. The cytotoxic activity on cultured human cancer cell lines was determined by MTT assay. Results: The inhibition of cobra venom cytotoxin on SGC-7901, Bel-7402, K562 and U937 showed a good dose-effect relationship, and the IC_(50) was 4. 10, 2. 08, 0. 29 and 0. 17 μg/ml, respectively. Conclusion: The cobra venom cytotoxin had a high cytotoxic activity on cultured human cancer cell lines.

关 键 词：眼镜蛇毒 细胞毒素 肿瘤细胞 抗癌活性 

分 类 号：R979.1[医药卫生—药品]