高效液相色谱-质谱法同时测定川芎提取液中7种有效成分  

Simultaneous determination of 7 kinds of bioactive components of Ligusticum chuanxiong Hort. by HPLC-MS

作　　者：刘洁[1] 余李敏[1] 翟帆[1] 

机构地区：[1]陕西新药技术开发中心,陕西西安710075

出　　处：《中成药》2010年第8期1380-1382,共3页Chinese Traditional Patent Medicine

摘　　要：目的:建立高效液相色谱-电喷雾质谱方法,测定川芎中香草醛等7种有效成分的含量。方法:超声法提取川芎中的有效成分,反相高效液相色谱梯度洗脱法进行分离,电喷雾质谱多级反应模式进行含量测定。结果:香草醛等7种有效成分的检测限分别为:40,20,80,20,10,60和50ng/mL,线性范围分别为:1～450,4～490,1～625,2～360,2～700,1～244和21～210μg/mL,在川芎药材中的平均含量分别为:0.12,0.25,0.41,0.99,0.30,8.96和11.25mg/g。结论:本方法简便、快速、灵敏度高,可作为川芎药材的多成分分析方法。AIM： To establish an HPLC-MS for determining seven bioactive components,including vanillin ferulaic acid,ligustrazine,senkyunolid I,H,and A,and ligustilide in Ligusticum chuanxiong Hort. . METHODS： The bioactive components were extracted by ultrasonic method,and then were separated by reverse HPLC,and finally were determined by the multiple reaction mode of MS. RESULTS： The detected limits of the seven bioactive components such as vanillin were 40,20,80,20,10,60 and 50 ng/mL,respectively. Their linear range were 1450,4-490,1-625,2-360,2-700,1-244 and 21-210 μg/mL,respectively. The average contents of the above components in Ligusticum chuanxiong Hort. were 0. 12,0. 25,0. 41,0. 99,0. 30,8. 96 and 11. 25 mg/g,respectively. CONCLUSION： The proposed method has the characteristic of simplicity,rapidty and high sensitivity. It could be used in analyzing the whole components of Ligusticum chuanxiong Hort. .

关 键 词：川芎 香草醛 阿魏酸 HPLC/MS 

分 类 号：R284.1[医药卫生—中药学]