SRY基因和MCM158、MAF45鉴定哺乳动物性别  

Study on sex-identification of mammalian using SRY and MCM158,MAF45

作　　者：张秀华[1] 李延兰[1] 吴登俊[2] 

机构地区：[1]新乡医学院生命科学技术系,河南新乡453003 [2]四川农业大学动物科技学院,四川雅安625014

出　　处：《黑龙江畜牧兽医》2010年第8期4-6,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：德国联邦科技与教育部(BMBF)资助项目(CHN00316)

摘　　要：为了研究SRY基因和Y染色体上微卫星标记对性别鉴定的影响,试验以哺乳动物为研究对象,采用多重PCR技术扩增绵羊和牛基因组中Y染色体上的2个微卫星标记和SRY基因,根据其基因型进行性别鉴定,试图通过一次DNA扩增同时提供性别鉴定和基因分型的信息。结果表明:所设计的SRY基因引物具有高度特异性,是性别鉴定的主要依据;Y染色体上的MCM158、MAF45两标记由于特异性不好而不适用于性别鉴定。To study the effect of the SRY gent and mierosatellite markers on Y chromosome on the sex identification, mammal were used as the research object in this experiment, The technique of multiple PCR was used to amplify two microsatellite markers on Y chromosome and SRY gene to identify sex by genotyping, tried to provide simultaneously information of the sex - identification and genotyping in one step. The result showed that the SRY primers were highly specific and could be used as the main basis for sex - identification. But primer specificity was not good for Y chromosome markers MCM158 and MAF45, and therefore these markers were not suited for sex identification.

关 键 词：SRY基因 微卫星标记 性别鉴定 

分 类 号：S814.7[农业科学—畜牧学]