藜草花叶病毒外壳蛋白基因的克隆与RT-PCR检测  

Cloning of coat protein gene and RT-PCR detection of Sowbane mosaic virus

作　　者：陈青[1] 李桂芬[2] 董菁[1] 陈红运[1] 廖富荣[1] 陈洪俊[2] 朱水芳[2] 

机构地区：[1]厦门出入境检验检疫局,厦门361026 [2]中国检验检疫科学研究院,北京100029

出　　处：《植物病理学报》2010年第4期438-441,共4页Acta Phytopathologica Sinica

基　　金：国家科技支撑计划项目(2006BAD08A13);公益性行业科研专项(200810632)

摘　　要：The nucleotide sequence of coat protein(cp)gene of Sowbane mosaic virus(SoMV)was determined.The cp gene of SoMV consists of 726 nucleotides and encodes a putative protein of 241 amino acid re-sidues.Sequence comparison showed that SoMV was most closely related to Rubus chlorotic mottle virus compared to other sobemoviruses.A primer pair was designed for the detection of SoMV based upon the determined cp nucleotide sequence.An expected fragment with 510 bp could be obtained from SoMV,while no specific band was observed from the healthy control.The result showed that the RT-PCR assay with the primer pair was suitable for the specific detection of SoMV.The nucleotide sequence of coat protein（cp）gene of Sowbane mosaic virus（SoMV）was determined.The cp gene of SoMV consists of 726 nucleotides and encodes a putative protein of 241 amino acid re-sidues.Sequence comparison showed that SoMV was most closely related to Rubus chlorotic mottle virus compared to other sobemoviruses.A primer pair was designed for the detection of SoMV based upon the determined cp nucleotide sequence.An expected fragment with 510 bp could be obtained from SoMV,while no specific band was observed from the healthy control.The result showed that the RT-PCR assay with the primer pair was suitable for the specific detection of SoMV.

关 键 词：RT-PCR检测 花叶病毒 外壳蛋白基因 克隆 藜 MOSAIC 花粉传播 种子传播 

分 类 号：S662.5[农业科学—果树学]